SAE2 / UBA2 Recombinant Rabbit Monoclonal Antibody [JM66-53]
cat.: ET1705-73
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JM66-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SAE2 / UBA2 aa 39-88 / 640. |
| Positive control: | HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, K-562 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse colon tissue, rat colon tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IP IHC-P |
1:5,000 1:10-1:50 1:50-1:200 |
| Uniprot #: | SwissProt: Q9UBT2 Human | Q9Z1F9 Mouse | B1WC32 Rat |
| Alternative names: | Anthracycline associated resistance ARX Anthracycline-associated resistance ARX ARX FLJ13058 HRIHFB2115 SAE 2 SAE2 SAE2_HUMAN SUMO 1 activating enzyme subunit 2 SUMO activating enzyme subunit 2 SUMO-activating enzyme subunit 2 UBA2 UBA2 ubiquitin activating enzyme E1 homolog Ubiquitin like 1 activating enzyme E1B Ubiquitin like modifier activating enzyme 2 Ubiquitin-like 1-activating enzyme E1B UBLE1B |
Images
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Fig1:
Western blot analysis of SAE2 / UBA2 on different lysates with Rabbit anti-SAE2 / UBA2 antibody (ET1705-73) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HT-29 cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 71 kDa Observed band size: 90 kDa Exposure time: 13 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-73) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-SAE2 / UBA2 antibody (ET1705-73) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-SAE2 / UBA2 antibody (ET1705-73) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.