DNA Ligase IV Recombinant Rabbit Monoclonal Antibody [JM64-32]
cat.: ET1705-77
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JM64-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 104 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DNA Ligase IV aa 484-705 / 911. |
| Positive control: | Jurkat cell lysate, human colon carcinoma tissue, human placenta tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:2,000 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: P49917 Human |
| Alternative names: | DNA ligase IV ATP dependent DNA joinase DNA ligase 4 DNA ligase IV DNA repair enzyme DNLI4_HUMAN LIG 4 LIG4 LIG4S Ligase IV Ligase IV DNA ATP dependent Polydeoxyribonucleotide synthase [ATP] 4 Polydeoxyribonucleotide synthase 4 Polydeoxyribonucleotide synthase Polynucleotide ligase Sealase |
Images
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Fig1:
Western blot analysis of DNA Ligase IV on Jurkat cell lysate with Rabbit anti-DNA Ligase IV antibody (ET1705-77) at 1:500 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1705-77, 1/500 in 5% BSA, room temperature for 2 hours Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/200,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 104 kDa Observed band size: 104 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-DNA Ligase IV antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-77, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DNA Ligase IV antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-77, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.