Epac1 Recombinant Rabbit Monoclonal Antibody [JM63-29]
cat.: ET1705-79
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IP, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JM63-29 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 104 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Epac1 aa 874-923 / 923. |
| Positive control: | Mouse kidney tissue lysates, PC-3 cell lysates, human prostate tissue, human stomach carcinoma tissue, mouse kidney tissue. |
| Subcellular location: | Endomembrane system. |
| Recommended Dilutions:
WB IHC-P IP IF-Cell IF-Tissue |
1:500-1:1,000 1:50-1:200 1:10-1:50 1:10-1:50 1:10-1:50 |
| Uniprot #: | SwissProt: O95398 Human | Q8VCC8 Mouse |
| Alternative names: | bcm910 CAMP GEFI cAMP regulated guanine nucleotide exchange factor I CAMPGEFI CGEF 1 CGEF1 EPA1 Epac 1 EPAC EPAC1 Exchange factor directly activated by cAMP 1 Exchange protein directly activated by cAMP 1 MGC21410 RAP guanine nucleotide exchange factor Rap guanine nucleotide exchange factor (GEF) 3 RAP guanine nucleotide exchange factor 3 Rap1 guanine nucleotide exchange factor directly activated by cAMP RAPGEF3 |
Images
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Fig1:
Western blot analysis of Epac1 on mouse kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-79, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 104 kDa Observed band size: 100 kDa |
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Fig2:
Western blot analysis of Epac1 on PC-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-79, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 104 kDa Observed band size: 95 kDa |
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Fig3: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Epac1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-79, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Epac1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-79, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Epac1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-79, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.