Epac1 Recombinant Rabbit Monoclonal Antibody [JM63-29]
cat.: ET1705-79
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Tissue
Clonality: Monoclonal
Clone number: JM63-29
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 104 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Epac1 aa 874-923 / 923.
Positive control: HUVEC cell lysate, mouse kidney tissue lysate, rat brain tissue lysate, human prostate tissue, human stomach carcinoma tissue, mouse kidney tissue.
Subcellular location: Endomembrane system.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
1:1,000
1:50-1:200
1:10-1:50
Uniprot #: SwissProt: O95398 Human | Q8VCC8 Mouse | Q9Z1C8 Rat
Alternative names: bcm910 CAMP GEFI cAMP regulated guanine nucleotide exchange factor I CAMPGEFI CGEF 1 CGEF1 EPA1 Epac 1 EPAC EPAC1 Exchange factor directly activated by cAMP 1 Exchange protein directly activated by cAMP 1 MGC21410 RAP guanine nucleotide exchange factor Rap guanine nucleotide exchange factor (GEF) 3 RAP guanine nucleotide exchange factor 3 Rap1 guanine nucleotide exchange factor directly activated by cAMP RAPGEF3
Images
ET1705-79_1.jpg Fig1: Western blot analysis of Epac1 on different lysates with Rabbit anti-Epac1 antibody (ET1705-79) at 1/1,000 dilution.

Lane 1: HUVEC cell lysate (20 µg/Lane)
Lane 2: Mouse kidney tissue lysate (40 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 104 kDa
Observed band size: 95 kDa

Exposure time: 40 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-79) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1705-79_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Epac1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-79, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-79_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Epac1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-79, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-79_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Epac1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-79, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.