HuR / ELAVL1 Recombinant Rabbit Monoclonal Antibody [JM96-33]
cat.: ET1705-81
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JM96-33 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HuR / ELAVL1 aa 50-99 / 326. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, HEK-293 (Human embryonic kidney cell) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, Mouse kidney tissue lysate, Rat brain tissue lysate, human cervix cancer tissue, mouse heart tissue, rat brain tissue, HeLa. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IHC-P IP IF-Cell |
1:500-1:2,000 1:100-1:1,000 1-2μg/sample 1:100 |
| Uniprot #: | SwissProt: Q15717 Human | P70372 Mouse | B5DF91 Rat |
| Alternative names: | ELAV (embryonic lethal abnormal vision Drosophila) like 1 ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) ELAV like 1 ELAV like RNA binding protein 1 ELAV-like protein 1 ELAV1 ELAV1_HUMAN Elavl1 Embryonic lethal abnormal vision drosophila homolog like 1 Hu Antigen R Hu-antigen R Hua HuR MelG |
Images
|
Fig1:
Western blot analysis of HuR / ELAVL1 on different lysates with Rabbit anti-HuR / ELAVL1 antibody (ET1705-81) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: HEK-293 (Human embryonic kidney cell) cell lysate Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 4: Mouse kidney tissue lysate Lane 5: Rat brain tissue lysate Cell Lysates at 15 µg/Lane, Tissue Lysates at 20 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1705-81, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 36 kDa Observed band size: 34 kDa |
|
Fig2:
Western blot analysis of HuR / ELAVL1 on different lysates with Rabbit anti-HuR / ELAVL1 antibody (ET1705-81) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si HuR / ELAVL1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 34 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-81) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-HuR / ELAVL1 antibody (ET1705-81) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-81) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-HuR / ELAVL1 antibody (ET1705-81) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-81) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-HuR / ELAVL1 antibody (ET1705-81) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-81) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunocytochemistry analysis of HeLa cells labeling HuR / ELAVL1 with Rabbit anti-HuR / ELAVL1 antibody (ET1705-81) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HuR / ELAVL1 antibody (ET1705-81) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig7:
HuR / ELAVL1 was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1705-81 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1705-81 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: ET1705-81 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of ET1705-81 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.