HuR ELAVL1 Recombinant Rabbit Monoclonal Antibody [JM96-33]
cat.: ET1705-81
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JM96-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HuR ELAVL1 aa 50-99 / 326. |
| Positive control: | Rat spleen tissue lysates, Rat heart tisse lysates, PC-12 cell lysates, Mouse heart tissue lysates, Hela cell lysates, Jurkat, rat lung tissue, human colon cancer tissue, human breast tissue, mouse testis tissue, mouse heart tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:500-1:2,000 1:100-1:500 1:50-1:100 1:10-1:50 |
| Uniprot #: | SwissProt: Q15717 Human | P70372 Mouse | B5DF91 Rat |
| Alternative names: | ELAV (embryonic lethal abnormal vision Drosophila) like 1 ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) ELAV like 1 ELAV like RNA binding protein 1 ELAV-like protein 1 ELAV1 ELAV1_HUMAN Elavl1 Embryonic lethal abnormal vision drosophila homolog like 1 Hu Antigen R Hu-antigen R Hua HuR MelG |
Images
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Fig1:
Western blot analysis of HuR/ELAVL1 on different lysates using anti-HuR/ELAVL1 antibody at 1/1,000 dilution. Positive control: Lane 1: Rat spleen tissue lysates Lane 2: Rat heart tisse lysates Lane 3: PC-12 cell lysates Lane 4: Mouse heart tissue lysates Lane 5: Hela cell lysates |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Jurkat cells with HuR/ELAVL1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.