Cullin 1 Recombinant Rabbit Monoclonal Antibody [JM72-30]
cat.: ET1705-82
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IHC-P, IP, FC, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JM72-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 90 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cullin 1 aa 727-776 / 776. |
| Positive control: | Hela cell lysate, MCF-7 cell lysates, rat brain tissue, human stomach carcinoma tissue, human fallopian tube tissue, human gallbladder tissue, Hela. |
| Subcellular location: | Cytoplasm, Nucleoplasm. |
| Recommended Dilutions:
WB IHC-P FC IP IF-Cell IF-Tissue |
1:500-1:2,000 1:50-1:200 1:50-1:100 1:10-1:50 1:10-1:50 1:10-1:50 |
| Uniprot #: | SwissProt: Q13616 Human | Q9WTX6 Mouse | F7FMJ3 Rat |
| Alternative names: | CUL 1 CUL-1 CUL1 CUL1_HUMAN Cullin-1 Cullin1 MGC149834 MGC149835 |
Images
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Fig1:
Western blot analysis of Cullin 1 on different lysates with Rabbit anti-Cullin 1 antibody (ET1705-82) at 1/2,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: MCF7 (Human breast cancer cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1705-82, 1/2,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 90 kDa Observed band size: 90 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cullin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Cullin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using anti-Cullin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-Cullin 1 antibody (ET1705-82) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-82) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of Cullin 1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-82, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.