Cullin 1 Recombinant Rabbit Monoclonal Antibody [JM72-30]
cat.: ET1705-82
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat, Mouse
Applications: WB, IHC-P, IP, FC, IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: JM72-30
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 90 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Cullin 1 aa 727-776 / 776.
Positive control: MCF-7 cell lysates, rat brain tissue, human stomach carcinoma tissue, human fallopian tube tissue, human gallbladder tissue, Hela.
Subcellular location: Cytoplasm, Nucleoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IP
  IF-Cell
  IF-Tissue
1:500-1:2,000
1:50-1:200
1:50-1:100
1:10-1:50
1:10-1:50
1:10-1:50
Uniprot #: SwissProt: Q13616 Human | Q9WTX6 Mouse | F7FMJ3 Rat
Alternative names: CUL 1 CUL-1 CUL1 CUL1_HUMAN Cullin-1 Cullin1 MGC149834 MGC149835
Images
ET1705-82_1.jpg Fig1: Western blot analysis of Cullin 1 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-82, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1705-82_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cullin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-82_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Cullin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-82_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using anti-Cullin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-82_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-Cullin 1 antibody (ET1705-82) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-82) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-82_6.jpg Fig6: Flow cytometric analysis of Cullin 1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-82, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.