Galectin 1 Recombinant Rabbit Monoclonal Antibody [JM13-37]
cat.: ET1705-83
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JM13-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Galectin 1 aa 1-50 / 135. |
| Positive control: | HL-60 cell lysate, LNCaP cell lysate, A549 cell lysate, HCT 116 cell lysate, A431, rat stomach tissue, human placenta tissue, NIH/3T3. |
| Subcellular location: | Cytoplasm, Extracellular matrix, Secreted. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:2,000 1:50-1:200 1:50-1:200 1:400-1:800 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P09382 Human | P16045 Mouse | P11762 Rat |
| Alternative names: | 14 kDa laminin-binding protein 14 kDa lectin Beta galactoside binding lectin Beta galactoside binding lectin L 14 I beta galactoside binding protein Beta-galactoside-binding lectin L-14-I DKFZp686E23103 Gal 1 Gal-1 GAL1 Galaptin Galbp Galectin Galectin-1 Galectin1 GBP HBL HLBP14 HPL L 14.5 L-14.5 L14 Lactose binding lectin 1 Lactose-binding lectin 1 Lect14 Lectin galactoside binding soluble 1 Lectin galactoside-binding soluble 1 LEG1_HUMAN LGALS 1 LGALS1 Lgals1 lectin galactose binding soluble 1 MAPK activating protein MP12 Putative MAPK activating protein MP12 Putative MAPK-activating protein PM12 S Lac lectin 1 S-Lac lectin 1 |
Images
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Fig1:
Western blot analysis of Galectin 1 on different lysates with Rabbit anti-Galectin 1 antibody (ET1705-83) at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: LNCaP cell lysate (negative) Lane 3: A549 cell lysate Lane 4: HCT 116 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 15 kDa Observed band size: 14 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-83) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Galectin 1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-83, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-Galectin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-83, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Galectin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-83, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of Galectin 1 was done on NIH/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-83, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.