GCLM Recombinant Rabbit Monoclonal Antibody [JM39-61]
cat.: ET1705-87
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P, IP, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JM39-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GCLM aa 225-274 / 274. |
| Positive control: | A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HeLa cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, human lung tissue, mouse liver tissue, rat liver tissue. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP IHC-Fr |
1:1,000-1:2,000 1:50-1:200 1:200-1:1,000 Use at an assay dependent concentration. 1:50 |
| Uniprot #: | SwissProt: P48507 Human | O09172 Mouse | P48508 Rat |
| Alternative names: | Gamma ECS regulatory subunit Gamma-ECS regulatory subunit Gamma-glutamylcysteine synthetase regulatory subunit GCLM GCS light chain GLCLR Glutamate cysteine ligase regulatory subunit Glutamate--cysteine ligase modifier subunit Glutamate--cysteine ligase regulatory subunit GSC light chain GSH0_HUMAN |
Images
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Fig1:
Western blot analysis of GCLM on different lysates with Rabbit anti-GCLM antibody (ET1705-87) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-87) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of GCLM on different lysates with Rabbit anti-GCLM antibody (ET1705-87) at 1/2,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: Mouse liver tissue lysate (20 µg/Lane) Lane 3: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-87) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling GCLM with Rabbit anti-GCLM antibody (ET1705-87). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1705-87, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
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Fig4:
Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling GCLM with Rabbit anti-GCLM antibody (ET1705-87). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1705-87, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GCLM antibody (ET1705-87) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-87) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-GCLM antibody (ET1705-87) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-87) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-GCLM antibody (ET1705-87) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-87) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-GCLM antibody (ET1705-87) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-87) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.