Klotho Recombinant Rabbit Monoclonal Antibody [JM93-76]
cat.: ET1705-88
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JM93-76
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 116 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Klotho aa 404-453 / 1,012.
Positive control: Human kidney tissue lysates, Human serum lysate, human kidney tissue lysate, human kidney tissue, mouse kidney tissue.
Subcellular location: Cell membrane. Apical cell membrane. Secreted.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:5,000
1:50-1:200
Uniprot #: SwissProt: Q9UEF7 Human | O35082 Mouse
Alternative names: Kl KLOT_HUMAN Klotho peptide
Images
ET1705-88_1.jpg Fig1: Western blot analysis of Klotho on human kidney tissue lysates with Rabbit anti-Klotho antibody (ET1705-88) at 1/5,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 116 kDa
Observed band size: 61 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-88) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1705-88_2.jpg Fig2: Western blot analysis of Klotho on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human serum lysate
Lane 2: Human kidney tissue lysate
ET1705-88_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Klotho antibody (ET1705-88) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-88) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-88_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Klotho antibody (ET1705-88) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-88) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.