OPA1 Recombinant Rabbit Monoclonal Antibody [JM81-35]
cat.: ET1705-9
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: JM81-35
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 112 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human OPA1 aa 896-945 / 960.
Positive control: Mouse testis tissue lysate, PC-12 cell lysate, mouse brain tissue lysate, Hela, SH-SY5Y, SW480, rat skeletal muscle tissue, human thyroid tissue, human kidney tissue, mouse brain tissue.
Subcellular location: Mitochondrion inner membrane. Mitochondrion intermembrane space.
Recommended Dilutions:
  WB
  IF-Tissue
  IHC-P
  FC
1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O60313 Human | P58281 Mouse | Q2TA68 Rat
Alternative names: Dynamin like 120 kDa protein Dynamin like 120 kDa protein, mitochondrial Dynamin-like 120 kDa protein, form S1 FLJ12460 Juvenile kjer type optic atrophy KIAA0567 KJER type Large GTP binding protein largeG MGM1 Mitochondrial dynamin like 120 kDa protein Mitochondrial dynamin like GTPase NPG NTG OAK OPA 1 opa1 OPA1 gene OPA1_HUMAN Optic atrophy 1 (autosomal dominant) OPTIC ATROPHY 1 Optic atrophy 1 gene protein Optic atrophy 1 homolog (human) Optic atrophy protein 1 Optic atrophy protein 1 homolog
Images
ET1705-9_1.jpg Fig1: Western blot analysis of OPA1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-9, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse testis tissue lysate
Lane 2: PC-12 cell lysate
Lane 3: mouse brain tissue lysate
ET1705-9_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-OPA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-9, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-9_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-OPA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-9, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-9_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-OPA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-9, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-9_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-OPA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-9, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-9_6.jpg Fig6: Flow cytometric analysis of OPA1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-9, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.