PSD93 Recombinant Rabbit Monoclonal Antibody [JM41-04]
cat.: ET1705-90
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JM41-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 98 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PSD93 aa 600-660. |
| Positive control: | Hela cell lysate, SH-SY5Y cell lysate, Caco-2 cell lysate, Pig brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, SH-SY5Y. |
| Subcellular location: | Cell junction, Cell membrane, Cell projection, Membrane, Synapse. |
| Recommended Dilutions:
WB FC IP |
1:1,000-1:5,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q15700 Human | Q91XM9 Mouse | Q63622 Rat |
| Alternative names: | Channel associated protein of synapse 110 Channel associated protein of synapses 110kD Channel-associated protein of synapse-110 Chapsyn 110 Chapsyn-110 Chapsyn110 Discs, large homolog 2 (Drosophila) Disks large homolog 2 DKFZp781D1854 DKFZp781E0954 Dlg 2 dlg2 DLG2_HUMAN Dlgh 2 Dlgh2 FLJ37266 Gm1197 MGC131811 Postsynaptic density protein PSD 93 Postsynaptic density protein PSD-93 Postsynaptic density protein PSD93 PSD 93 PSD93 |
Images
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Fig1:
Western blot analysis of PSD93 on different lysates with Rabbit anti-PSD93 antibody (ET1705-90) at 1/5,000 dilution. Lane 1: HeLa cell lysate, 15 µg/Lane Lane 2: SH-SY5Y cell lysate, 15 µg/Lane Lane 3: Caco-2 cell lysate, 15 µg/Lane Lane 4: Pig brain tissue lysate, 30 µg/Lane Lane 5: Mouse brain tissue lysate, 30 µg/Lane Lane 6: Rat brain tissue lysate, 30 µg/Lane Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1705-90, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 97.6 kDa Observed band size: 110 kDa |
|
Fig2: Flow cytometric analysis of PSD93 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.