PSD93 Recombinant Rabbit Monoclonal Antibody [JM41-04]
cat.: ET1705-90
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC, IP
Clonality: Monoclonal
Clone number: JM41-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 98 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human PSD93 aa 600-660.
Positive control: Hela cell lysate, SH-SY5Y cell lysate, SH-SY5Y.
Subcellular location: Cell junction, Cell membrane, Cell projection, Membrane, Synapse.
Recommended Dilutions:
  WB
  FC
  IP

1:500-1:2,000
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q15700 Human | Q91XM9 Mouse | Q63622 Rat
Alternative names: Channel associated protein of synapse 110 Channel associated protein of synapses 110kD Channel-associated protein of synapse-110 Chapsyn 110 Chapsyn-110 Chapsyn110 Discs, large homolog 2 (Drosophila) Disks large homolog 2 DKFZp781D1854 DKFZp781E0954 Dlg 2 dlg2 DLG2_HUMAN Dlgh 2 Dlgh2 FLJ37266 Gm1197 MGC131811 Postsynaptic density protein PSD 93 Postsynaptic density protein PSD-93 Postsynaptic density protein PSD93 PSD 93 PSD93
Images
ET1705-90_1.jpg Fig1: Western blot analysis of PSD93 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: SH-SY5Y cell lysate
ET1705-90_2.jpg Fig2: Flow cytometric analysis of PSD93 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.