Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, FC, IP |
Clonality: | Monoclonal |
Clone number: | JM41-04 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 98 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human PSD93 aa 600-660. |
Positive control: | Hela cell lysate, SH-SY5Y cell lysate, SH-SY5Y. |
Subcellular location: | Cell junction, Cell membrane, Cell projection, Membrane, Synapse. |
Recommended Dilutions:
WB FC IP |
1:500-1:2,000 1:50-1:100 Use at an assay dependent concentration. |
Uniprot #: | SwissProt: Q15700 Human | Q91XM9 Mouse | Q63622 Rat |
Alternative names: | Channel associated protein of synapse 110 Channel associated protein of synapses 110kD Channel-associated protein of synapse-110 Chapsyn 110 Chapsyn-110 Chapsyn110 Discs, large homolog 2 (Drosophila) Disks large homolog 2 DKFZp781D1854 DKFZp781E0954 Dlg 2 dlg2 DLG2_HUMAN Dlgh 2 Dlgh2 FLJ37266 Gm1197 MGC131811 Postsynaptic density protein PSD 93 Postsynaptic density protein PSD-93 Postsynaptic density protein PSD93 PSD 93 PSD93 |
Fig1:
Western blot analysis of PSD93 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SH-SY5Y cell lysate |
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Fig2: Flow cytometric analysis of PSD93 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |