Aldolase Recombinant Rabbit Monoclonal Antibody [JM54-19]
cat.: ET1705-91
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JM54-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Aldolase . |
| Positive control: | A549 cell lysate, HeLa cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, Rat spleen tissue lysate, zebrafish tissue lysates, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:1,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P04075 Human | P05064 Mouse | P05065 Rat |
| Alternative names: | ALDA Aldo1 ALDOA ALDOA_HUMAN Aldolase 1 Aldolase A Aldolase A fructose bisphosphatase Aldolase A fructose bisphosphate Aldolase, fructose-bisphosphate A Epididymis secretory sperm binding protein Li 87p FRUCTOALDOLASE A Fructose 1 6 bisphosphate triosephosphate lyase Fructose bisphosphate aldolase A Fructose bisphosphate aldolase FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A Fructose-bisphosphate aldolase A Fructose-bisphosphate aldolase A Muscle-type GSD12 HEL S 87p Lung cancer antigen NY LU 1 Lung cancer antigen NY-LU-1 MGC10942 MGC17716 MGC17767 Muscle type aldolase Muscle-type aldolase RNALDOG5 |
Images
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Fig1:
Western blot analysis of Aldolase on different lysates with Rabbit anti-Aldolase antibody (ET1705-91) at 1/1,000 dilution. Lane 1: A549 cell lysate (10 µg/Lane) Lane 2: HeLa cell lysate (10 µg/Lane) Lane 3: Mouse liver tissue lysate (20 µg/Lane) Lane 4: Rat liver tissue lysate (20 µg/Lane) Lane 5: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-91) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of Aldolase on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Aldolase antibody (ET1705-91) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-91) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Aldolase antibody (ET1705-91) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-91) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Aldolase antibody (ET1705-91) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-91) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.