Aldolase Recombinant Rabbit Monoclonal Antibody [JM54-19]
cat.: ET1705-91
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IHC-P, IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: JM54-19
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human Aldolase .
Positive control: A549 cell lysate, Hela cell lysate, mouse liver tissue lysate, rat liver tissue lysate, rat spleen tissue lysate, zebrafish tissue lysates, A431, Hela, SK-Br-3, rat skeletal muscle tissue, human fetal skeletal muscle tissue, human pancreas tissue, mouse brain tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P04075 Human | P05064 Mouse | P05065 Rat
Alternative names: ALDA Aldo1 ALDOA ALDOA_HUMAN Aldolase 1 Aldolase A Aldolase A fructose bisphosphatase Aldolase A fructose bisphosphate Aldolase, fructose-bisphosphate A Epididymis secretory sperm binding protein Li 87p FRUCTOALDOLASE A Fructose 1 6 bisphosphate triosephosphate lyase Fructose bisphosphate aldolase A Fructose bisphosphate aldolase FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A Fructose-bisphosphate aldolase A Fructose-bisphosphate aldolase A Muscle-type GSD12 HEL S 87p Lung cancer antigen NY LU 1 Lung cancer antigen NY-LU-1 MGC10942 MGC17716 MGC17767 Muscle type aldolase Muscle-type aldolase RNALDOG5
Images
ET1705-91_1.jpg Fig1: Western blot analysis of Aldolase on different lysates with Rabbit anti-Aldolase antibody (ET1705-91) at 1/1,000 dilution.

Lane 1: A549 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: Mouse liver (20 µg/Lane)
Lane 4: Rat liver (20 µg/Lane)
Lane 5: Rat spleen (20 µg/Lane)

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 1 minute 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-91) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET1705-91_2.jpg Fig2: Western blot analysis of Aldolase on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1705-91_3.jpg Fig3: ICC staining of Aldolase in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-91, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1705-91_4.jpg Fig4: ICC staining of Aldolase in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-91, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1705-91_5.jpg Fig5: ICC staining of Aldolase in SK-Br-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-91, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1705-91_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Aldolase antibody (ET1705-91) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-91) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-91_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Aldolase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-91, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-91_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Aldolase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-91, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-91_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Aldolase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-91, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.