Anti-TLR2 antibody [JM22-41]
cat.: ET1705-92
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, FC
Clonality: Monoclonal
Clone number: JM22-41
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 90 kDa
Isotype: IgG
Immunogen: Recombinant full length protein of human TLR2.
Positive control: PC-3M, A549, HepG2, mouse spleen tissue, human spleen tissue, THP-1.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O60603 Human | Q9QUN7 Mouse
Alternative names: CD282 antibody CD282 antigen antibody TIL 4 antibody TIL4 antibody TLR 2 antibody TLR2 antibody TLR2_HUMAN antibody Toll like receptor 2 antibody Toll like receptor 2 precursor antibody Toll-like receptor 2 antibody Toll/interleukin 1 receptor like 4 antibody Toll/interleukin 1 receptor like protein 4 antibody Toll/interleukin receptor like protein 4 antibody Toll/interleukin-1 receptor-like protein 4 antibody
Images
ET1705-92_1.jpg Fig1: ICC staining of TLR2 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-92, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1705-92_2.jpg Fig2: ICC staining of TLR2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-92, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1705-92_3.jpg Fig3: ICC staining of TLR2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-92, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1705-92_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-TLR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-92, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-92_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TLR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-92, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-92_6.jpg Fig6: Flow cytometric analysis of TLR2 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-92, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.