Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IHC-P, IF-Cell, IF-Tissue |
Clonality: | Monoclonal |
Clone number: | JM62-45 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 31 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human PDX1 234-283 / 283. |
Positive control: | Hela cell lysates, human pancreas tissue, human gallbladder tissue, human stomach tissue. |
Subcellular location: | Cytosol, Nucleus. |
Recommended Dilutions:
WB IHC-P IF-Cell IF-Tissue |
1:500-1:1,000 1:100-1:800 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: P52945 Human |
Alternative names: | Glucose sensitive factor Glucose-sensitive factor GSF IDX 1 IDX-1 IDX1 Insulin promoter factor 1 Insulin promoter factor 1 homeodomain transcription factor Insulin upstream factor 1 IPF 1 IPF-1 IPF1 Islet/duodenum homeobox 1 Islet/duodenum homeobox-1 IUF 1 IUF-1 IUF1 MODY4 Pancreas/duodenum homeobox 1 Pancreas/duodenum homeobox protein 1 pancreatic and duodenal homeobox P PDX 1 PDX-1 PDX1 PDX1_HUMAN Somatostatin transactivating factor 1 Somatostatin-transactivating factor 1 STF 1 STF-1 STF1 |
Fig1: Western blot analysis of PDX1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. | |
Fig2: Immunofluorescence staining of paraffin-embedded human pancreas tissue using anti-PDX1 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ET1705-99 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature. | |
Fig3:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PDX1 antibody (ET1705-99) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-99) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-PDX1 antibody (ET1705-99) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-99) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig5:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-PDX1 antibody (ET1705-99) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-99) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |