PDX1 Recombinant Rabbit Monoclonal Antibody [JM62-45]
cat.: ET1705-99
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: JM62-45
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 31 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PDX1 234-283 / 283.
Positive control: Hela cell lysates, human pancreas tissue, human gallbladder tissue, human stomach tissue.
Subcellular location: Cytosol, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  IF-Tissue
1:500-1:1,000
1:100-1:800
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P52945 Human
Alternative names: Glucose sensitive factor Glucose-sensitive factor GSF IDX 1 IDX-1 IDX1 Insulin promoter factor 1 Insulin promoter factor 1 homeodomain transcription factor Insulin upstream factor 1 IPF 1 IPF-1 IPF1 Islet/duodenum homeobox 1 Islet/duodenum homeobox-1 IUF 1 IUF-1 IUF1 MODY4 Pancreas/duodenum homeobox 1 Pancreas/duodenum homeobox protein 1 pancreatic and duodenal homeobox P PDX 1 PDX-1 PDX1 PDX1_HUMAN Somatostatin transactivating factor 1 Somatostatin-transactivating factor 1 STF 1 STF-1 STF1
Images
ET1705-99_1.jpg Fig1: Western blot analysis of PDX1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1705-99_2.jpg Fig2: Immunofluorescence staining of paraffin-embedded human pancreas tissue using anti-PDX1 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ET1705-99 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
ET1705-99_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PDX1 antibody (ET1705-99) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-99) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-99_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human gallbladder tissue with Rabbit anti-PDX1 antibody (ET1705-99) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-99) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-99_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-PDX1 antibody (ET1705-99) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-99) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1705-99_6.jpg Fig6: Immunocytochemistry analysis of HeLa cells labeling PDX1 with Rabbit anti-PDX1 antibody (ET1705-99) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PDX1 antibody (ET1705-99) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.