HP1 gamma Recombinant Rabbit Monoclonal Antibody [JU81-36]
cat.: ET1706-02
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JU81-36
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 21 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human HP1 gamma aa 38-87 / 183.
Positive control: HeLa, MCF7,NIH/3T3,C6,PC-12,mouse colon tissue lysates, rat testis tissue, human liver carcinoma tissue, human tonsil tissue, human placenta tissue, mouse colon tissue, mouse testis tissue, Hela.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IP
1:5,000
1:50-1:200
1:50-1:100
1:10-1:50
Uniprot #: SwissProt: Q13185 Human | P23198 Mouse | Q5RJK5 Rat
Alternative names: CBX 3 CBX3 CBX3_HUMAN Chromobox homolog 3 (HP1 gamma homolog, Drosophila) Chromobox homolog 3 Chromobox protein homolog 3 GAMMA HECH Heterochromatin like protein 1 Heterochromatin protein 1 homolog gamma Heterochromatin protein HP1 gamma HP1 gamma HP1 gamma homolog HP1Hs gamma Modifier 2 protein
Images
ET1706-02_1.jpg Fig1: Western blot analysis of HP1 gamma on different lysates with Rabbit anti-HP1 gamma antibody (ET1706-02) at 1/5,000 dilution.

Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: MCF7 (Human breast cancer cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 4: C6 (Rat glioma cell) cell lysate
Lane 5: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate

Cell Lysates at 15 µg/Lane
Exposure time: 60 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1706-02, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 21 kDa
Observed band size: 21 kDa
ET1706-02_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-HP1 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-02, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-02_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-HP1 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-02, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-02_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HP1 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-02, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-02_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HP1 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-02, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-02_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HP1 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-02, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-02_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HP1 gamma antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-02, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-02_8.jpg Fig8: Flow cytometric analysis of HP1 gamma was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.