Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IP |
Clonality: | Monoclonal |
Clone number: | JU83-16 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 119 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human Eg5 aa 860-1,056 / 1,056. |
Positive control: | A431 cell lysate, 293 cell lysate, Jurkat cell lysate. |
Subcellular location: | Cytoplasm, Cytoskeleton, Microtubule. |
Recommended Dilutions:
WB IP |
1:500-1:2,000 1:10-1:50 |
Uniprot #: | SwissProt: P52732 Human |
Alternative names: | EG5 HKSP KIF11 KIF11_HUMAN Kinesin family member 11 Kinesin like protein 1 Kinesin-like protein 1 Kinesin-like protein KIF11 Kinesin-like spindle protein HKSP Kinesin-related motor protein Eg5 KNSL1 MCLMR Thyroid receptor-interacting protein 5 TR-interacting protein 5 TRIP-5 TRIP5 |
Fig1:
Western blot analysis of Eg5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1706-03, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: 293 cell lysate Lane 2: Jurkat cell lysate |