Eg5 Recombinant Rabbit Monoclonal Antibody [JU83-16]
cat.: ET1706-03
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IP
Clonality: Monoclonal
Clone number: JU83-16
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 119 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Eg5 aa 860-1,056 / 1,056.
Positive control: A431 cell lysate, 293 cell lysate, Jurkat cell lysate.
Subcellular location: Cytoplasm, Cytoskeleton, Microtubule.
Recommended Dilutions:
  WB
  IP

1:500-1:2,000
1:10-1:50
Uniprot #: SwissProt: P52732 Human
Alternative names: EG5 HKSP KIF11 KIF11_HUMAN Kinesin family member 11 Kinesin like protein 1 Kinesin-like protein 1 Kinesin-like protein KIF11 Kinesin-like spindle protein HKSP Kinesin-related motor protein Eg5 KNSL1 MCLMR Thyroid receptor-interacting protein 5 TR-interacting protein 5 TRIP-5 TRIP5
Images
ET1706-03_1.jpg Fig1: Western blot analysis of Eg5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1706-03, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: 293 cell lysate
Lane 2: Jurkat cell lysate
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.