CD11b Recombinant Rabbit Monoclonal Antibody [JU93-81]
cat.: ET1706-04
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IP, IHC-P, FC, ICC/IF
Clonality: Monoclonal
Clone number: JU93-81
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 127 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD11b 1103-1152 / 1152.
Positive control: TF-1 cell lysates, LOVO, human lymph node tissue, human spleen tissue, human tonsil tissue, THP-1.
Subcellular location: Cell membrane, Membrane raft
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC
  IP

1:500-1:1,000
1:50-1:200
1:50-1:800
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P11215 Human
Alternative names: antigen CD11b (p170) Antigen CD11b p170 CD11 antigen like family member B CD11 antigen-like family member B CD11b CD11b/CD18 CD49d Cell surface glycoprotein MAC-1 subunit alpha Complement component 3 receptor 3 subunit Complement Component Receptor 3 Alpha Complement receptor type 3, alpha subunit CR 3 alpha chain (CR3A) CR 3 alpha chain CR-3 alpha chain CR3 CR3A F730045J24Rik Integrin Alpha M Integrin alpha M chain Integrin alpha-M Integrin beta 2 alpha subunit Integrin subunit alpha M integrin, alpha M (complement component 3 receptor 3 subunit) ITAM_HUMAN ITGAM Leukocyte adhesion receptor MO1 Ly-40 MAC 1 Mac-1a MAC1 Mac1, alpha subunit MAC1A Macrophage antigen alpha polypeptide MGC117044 Mo1, alpha subunit MO1A Neutrophil adherence receptor alpha M subunit Neutrophil adherence receptor SLEB6
Images
ET1706-04_1.jpg Fig1: Western blot analysis of CD11b on TF-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1706-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1706-04_2.jpg Fig2: ICC staining of CD11b in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1706-04, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1706-04_3.jpg Fig3: Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labelling CD11 b (ET1706-04).

The human lymph node section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes, blocked in 10% goat serum, and then incubated with ET1706-04 at 1/50 dilution , followed by iFluorTM 488 Conjugated Goat anti-rabbit IgG at 1:1000 dilution. Nuclear was stained with Hoechst 33258 at 1/5,000 dilution.
Confocal images shows specific membrane staining of CD11b in human lymph node.
ET1706-04_4.jpg Fig4: Immunofluorescence analysis of paraffin-embedded human spleen tissue labelling CD11 b (ET1706-04).

The human spleen section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes, blocked in 10% goat serum, and then incubated with ET1706-04 at 1/50 dilution , followed by iFluorTM 488 Conjugated Goat anti-rabbit IgG at 1:1000 dilution. Nuclear was stained with Hoechst 33258 at 1/5,000 dilution.
Confocal images shows specific membrane staining of CD11b in human spleen.
ET1706-04_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-04, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-04_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD11b antibody (ET1706-04) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-04) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-04_7.jpg Fig7: Flow cytometric analysis of CD11b was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-04, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1706-04_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD11b antibody (ET1706-04) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.