GluR2 Recombinant Rabbit Monoclonal Antibody [JU20-43]
cat.: ET1706-06
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, IP
Clonality: Monoclonal
Clone number: JU20-43
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 99 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human GluR2 aa 181-230 / 883.
Positive control: Rat brain lysates, N2A, SH-SY5Y, rat brain tissue, mouse brain tissue.
Subcellular location: Cell membrane, postsynaptic cell membrane, postsynaptic density membrane, endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  IP

1:500
1:50-1:200
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P42262 Human | P23819 Mouse | P19491 Rat
Alternative names: AMPA 2 AMPA selective glutamate receptor 2 AMPA-selective glutamate receptor 2 AMPA2 GluA2 GLUR 2 GLUR B GluR K2 GluR-2 GluR-B GluR-K2 GLUR2 GLURB Glutamate receptor 2 Glutamate receptor ionotropic AMPA 2 Glutamate receptor ionotropic Gria2 GRIA2_HUMAN HBGR2
Images
ET1706-06_1.jpg Fig1: Western blot analysis of GluR2 on rat brain lysates with Rabbit anti-GluR2 antibody (ET1706-06) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 99 kDa
Observed band size: 90 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-06) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1706-06_2.jpg Fig2: Immunocytochemistry analysis of N2A cells labeling GluR2 with Rabbit anti-GluR2 antibody (ET1706-06) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GluR2 antibody (ET1706-06) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-06_3.jpg Fig3: Immunocytochemistry analysis of SH-SY5Y cells labeling GluR2 with Rabbit anti-GluR2 antibody (ET1706-06) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GluR2 antibody (ET1706-06) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-06_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GluR2 antibody (ET1706-06) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-06) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-06_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GluR2 antibody (ET1706-06) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-06) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.