GluR2 Recombinant Rabbit Monoclonal Antibody [JU20-43]
cat.: ET1706-06
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU20-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 99 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GluR2 aa 181-230 / 883. |
| Positive control: | Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, N2A, SH-SY5Y, rat brain tissue, mouse brain tissue. |
| Subcellular location: | Cell membrane, postsynaptic cell membrane, postsynaptic density membrane, endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P42262 Human | P23819 Mouse | P19491 Rat |
| Alternative names: | AMPA 2 AMPA selective glutamate receptor 2 AMPA-selective glutamate receptor 2 AMPA2 GluA2 GLUR 2 GLUR B GluR K2 GluR-2 GluR-B GluR-K2 GLUR2 GLURB Glutamate receptor 2 Glutamate receptor ionotropic AMPA 2 Glutamate receptor ionotropic Gria2 GRIA2_HUMAN HBGR2 |
Images
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Fig1:
Western blot analysis of GluR2 on different lysates with Rabbit anti-GluR2 antibody (ET1706-06) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse heart tissue lysate (negative) Lane 4: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 99 kDa Observed band size: 99 kDa Exposure time: Lane 1: 3 minutes; Lane 3: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-06) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of N2A cells labeling GluR2 with Rabbit anti-GluR2 antibody (ET1706-06) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GluR2 antibody (ET1706-06) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of SH-SY5Y cells labeling GluR2 with Rabbit anti-GluR2 antibody (ET1706-06) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GluR2 antibody (ET1706-06) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GluR2 antibody (ET1706-06) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-06) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GluR2 antibody (ET1706-06) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-06) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.