NDUFS3 Recombinant Rabbit Monoclonal Antibody [JU10-32]
cat.: ET1706-07
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | JU10-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NDUFS3 aa 215-264 / 264. |
| Positive control: | HepG2 lysates, MCF-7, human colon carcinoma tissue, human kidney tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell IP |
1:500 1:50 1:50 1:10-1:50 |
| Uniprot #: | SwissProt: O75489 Human |
| Alternative names: | CI 30 CI 30KD CI-30kD Complex I 30KD Complex I 30kDa subunit COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT Complex I-30kD mitochondrial NADH coenzyme Q reductase NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 NADH dehydrogenase ubiquinone 30 kDa subunit NADH-ubiquinone oxidoreductase 30 kDa subunit NADH-Ubiquinone Oxidoreductase Fe-S Protein 3 NDUFS3 NDUS3_HUMAN |
Images
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Fig1:
Western blot analysis of NDUFS3 on HepG2 lysates with Rabbit anti-NDUFS3 antibody (ET1706-07) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 20 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-07) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of MCF-7 cells labeling NDUFS3 with Rabbit anti-NDUFS3 antibody (ET1706-07) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NDUFS3 antibody (ET1706-07) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of MCF-7 cells labeling NDUFS3 with Rabbit anti-NDUFS3 antibody (ET1706-07) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NDUFS3 antibody (ET1706-07) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NDUFS3 antibody (ET1706-07) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-07) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFS3 antibody (ET1706-07) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-07) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.