Fragilis Recombinant Rabbit Monoclonal Antibody [JU73-02]
cat.: ET1706-09
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JU73-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Fragilis aa 1-50 / 133. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, HUVEC, MCF-7, PC-3M, human breast carcinoma tissue, human colon carcinoma tissue. |
| Subcellular location: | Cell membrane, Late endosome membrane, Lysosome membrane, perinuclear region. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:2,000 1:50-1:200 1:50-1:200 1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q01628 Human |
| Alternative names: | 1 8U Fragilis IFITM3 IFM3_HUMAN Interferon induced transmembrane protein 3 (1 8U) Interferon Induced Transmembrane Protein 3 Interferon inducible Interferon inducible protein 1 8U Interferon Inducible Protein 15 Interferon Inducible Protein Homolog Interferon-induced transmembrane protein 3 Interferon-inducible protein 1-8U IP15 |
Images
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Fig1:
Western blot analysis of Fragilis on different lysates with Rabbit anti-Fragilis antibody (ET1706-09) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-09) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HUVEC cells labeling Fragilis with Rabbit anti-Fragilis antibody (ET1706-09) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Fragilis antibody (ET1706-09) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of MCF-7 cells labeling Fragilis with Rabbit anti-Fragilis antibody (ET1706-09) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Fragilis antibody (ET1706-09) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of PC-3M cells labeling Fragilis with Rabbit anti-Fragilis antibody (ET1706-09) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Fragilis antibody (ET1706-09) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Fragilis antibody (ET1706-09) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-09) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Fragilis antibody (ET1706-09) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-09) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunofluorescence analysis of paraffin-embedded human breast cancer tissue labeling Fragilis with Rabbit anti-Fragilis antibody (ET1706-09) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1706-09, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig8: Flow cytometric analysis of Fragilis was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-09, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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