USP22 Recombinant Rabbit Monoclonal Antibody [JU63-27]
cat.: ET1706-13
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JU63-27 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 60 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human USP22 aa 321-370 / 525. |
| Positive control: | Hela cell lysate, HepG2 cell lysate, Hela, LOVO, SH-SY5Y. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue FC IP |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:100 1:10-1:50 |
| Uniprot #: | SwissProt: Q9UPT9 Human |
| Alternative names: | Deubiquitinating enzyme 22 KIAA1063 Ubiquitin carboxyl terminal hydrolase 22 Ubiquitin carboxyl-terminal hydrolase 22 Ubiquitin specific peptidase 22 Ubiquitin specific peptidase 3 like Ubiquitin specific processing protease 22 Ubiquitin specific protease 22 Ubiquitin thioesterase 22 Ubiquitin thiolesterase 22 Ubiquitin-specific-processing protease 22 UBP22_HUMAN USP 22 Usp22 USP3L |
Images
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Fig1:
Western blot analysis of USP22 on different lysates with Rabbit anti-USP22 antibody (ET1706-13) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-13) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of Hela cells labeling USP22 with Rabbit anti-USP22 antibody (ET1706-13) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USP22 antibody (ET1706-13) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of LOVO cells labeling USP22 with Rabbit anti-USP22 antibody (ET1706-13) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USP22 antibody (ET1706-13) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
|
Fig4:
Immunocytochemistry analysis of SH-SY5Y cells labeling USP22 with Rabbit anti-USP22 antibody (ET1706-13) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USP22 antibody (ET1706-13) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig5: Flow cytometric analysis of Hela cells with USP22 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.