USP22 Recombinant Rabbit Monoclonal Antibody [JU63-27]
cat.: ET1706-13
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | JU63-27 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human USP22 aa 321-370 / 525. |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, MCF7 cell lysate, Neuro-2a cell lysate, F9 cell lysate, C6 cell lysate, PC-12 cell lysate, Human brain tissue lysate, Human liver tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IP |
1:1,000-1:5,000 1:200 1:50 1-2μg/sample |
| Uniprot #: | SwissProt: Q9UPT9 Human | Q5DU02 Mouse Entrez Gene: 303201 Rat |
| Alternative names: | Deubiquitinating enzyme 22 KIAA1063 Ubiquitin carboxyl terminal hydrolase 22 Ubiquitin carboxyl-terminal hydrolase 22 Ubiquitin specific peptidase 22 Ubiquitin specific peptidase 3 like Ubiquitin specific processing protease 22 Ubiquitin specific protease 22 Ubiquitin thioesterase 22 Ubiquitin thiolesterase 22 Ubiquitin-specific-processing protease 22 UBP22_HUMAN USP 22 Usp22 USP3L |
Images
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Fig1:
Western blot analysis of USP22 on different lysates with Rabbit anti-USP22 antibody (ET1706-13) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: Jurkat cell lysate (15 µg/Lane) Lane 4: MCF7 cell lysate (15 µg/Lane) Lane 5: Neuro-2a cell lysate (15 µg/Lane) Lane 6: F9 cell lysate (15 µg/Lane) Lane 7: C6 cell lysate (15 µg/Lane) Lane 8: PC-12 cell lysate (15 µg/Lane) Lane 9: Human brain tissue lysate (30 µg/Lane) Lane 10: Human liver tissue lysate (30 µg/Lane) Lane 11: Mouse brain tissue lysate (30 µg/Lane) Lane 12: Rat brain tissue lysate (30 µg/Lane) Lane 13: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: Lane 1-3: 6 seconds; Lane 4-13: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-13) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of USP22 on different lysates with Rabbit anti-USP22 antibody (ET1706-13) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-USP22 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 60 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-13) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-USP22 antibody (ET1706-13) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-13) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-USP22 antibody (ET1706-13) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-13) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling USP22 with Rabbit anti-USP22 antibody (ET1706-13) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1706-13, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig6:
USP22 was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1706-13 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1706-13 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: ET1706-13 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of ET1706-13 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 14 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.