USP13 Recombinant Rabbit Monoclonal Antibody [JU72-38]
cat.: ET1706-14
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JU72-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 97 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human USP13 aa 739-788 / 863. |
| Positive control: | Mouse testis tissue lysates, A431, Hela, SH-SY-5Y. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue FC IP |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 1:10-1:50 |
| Uniprot #: | SwissProt: Q92995 Human | Q5BKP2 Mouse |
| Alternative names: | Deubiquitinating enzyme 13 Isopeptidase T 3 Isopeptidase T-3 Isopeptidase T3 ISOT 3 ISOT-3 ISOT3 Ubiquitin carboxyl terminal hydrolase 13 Ubiquitin carboxyl-terminal hydrolase 13 Ubiquitin specific peptidase 13 (isopeptidase T3) Ubiquitin specific peptidase 13 Ubiquitin specific processing protease 13 Ubiquitin specific protease 13 Ubiquitin thioesterase 13 Ubiquitin thiolesterase 13 Ubiquitin-specific-processing protease 13 UBP13 UBP13_HUMAN USP 13 USP13 |
Images
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Fig1:
Western blot analysis of USP13 on mouse testis tissue lysates with Rabbit anti-USP13 antibody (ET1706-14) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-14) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of A431 cells labeling USP13 with Rabbit anti-USP13 antibody (ET1706-14) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USP13 antibody (ET1706-14) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of Hela cells labeling USP13 with Rabbit anti-USP13 antibody (ET1706-14) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USP13 antibody (ET1706-14) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
|
Fig4:
Immunocytochemistry analysis of SH-SY-5Y cells labeling USP13 with Rabbit anti-USP13 antibody (ET1706-14) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USP13 antibody (ET1706-14) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
|
Fig5: Flow cytometric analysis of SH-SY5Y cells with USP13 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.