Plexin A1 Recombinant Rabbit Monoclonal Antibody [JU50-37]
cat.: ET1706-17
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JU50-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 211 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Plexin A1 aa 100-200 / 1,896. |
| Positive control: | K-562 cell lysate, HUVEC cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9UIW2 Human | P70206 Mouse | D3Z981 Rat |
| Alternative names: | NOV NOV/plexin A1 protein NOVP Plexin-A1 Plexin1 PlexinA1 PLXA1_HUMAN PLXN1 Plxna1 PLXNA1 protein Semaphorin receptor NOV |
Images
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Fig1:
Western blot analysis of Plexin A1 on different lysates with Rabbit anti-Plexin A1 antibody (ET1706-17) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: HUVEC cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 211 kDa Observed band size: 250 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.