Plexin A1 Recombinant Rabbit Monoclonal Antibody [JU50-37]
cat.: ET1706-17
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JU50-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 211 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Plexin A1 aa 100-200 / 1,896. |
| Positive control: | Rat brain tissue lysates, LOVO. |
| Subcellular location: | Cell Membrane. |
| Recommended Dilutions:
IF-Cell FC WB |
1:50-1:200 1:50-1:100 1:500 |
| Uniprot #: | SwissProt: Q9UIW2 Human | P70206 Mouse | D3Z981 Rat |
| Alternative names: | NOV NOV/plexin A1 protein NOVP Plexin-A1 Plexin1 PlexinA1 PLXA1_HUMAN PLXN1 Plxna1 PLXNA1 protein Semaphorin receptor NOV |
Images
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Fig1:
Western blot analysis of Plexin A1 on rat brain tissue lysates with Rabbit anti-Plexin A1 antibody (ET1706-17) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 211 kDa Observed band size: 211 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-17) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of LOVO cells labeling Plexin A1 with Rabbit anti-Plexin A1 antibody (ET1706-17) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Plexin A1 antibody (ET1706-17) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3: Flow cytometric analysis of Plexin A1 was done on LOVO cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-17, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.