PSMA1 Recombinant Rabbit Monoclonal Antibody [JU66-24]
cat.: ET1706-19
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: JU66-24
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 30 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PSMA1 aa 214-263 / 263.
Positive control: Jurkat cell lysate, Hela cell lysate, HepG2 cell lysate, 293 cell lysate, PC-12 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, HepG2, PC-3M, human liver carcinoma tissue, rat testis tissue, mouse cerebellum tissue, human pancreas tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP
1:500-1:2,000
1:100-1:500
1:100-1:500
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P25786 Human | Q9R1P4 Mouse | P18420 Rat
Alternative names: 30 kDa prosomal protein HC 2 HC2 Macropain subunit C2 Macropain subunit nu MGC14542 MGC14575 MGC14751 MGC1667 MGC21459 MGC22853 MGC23915 Multicatalytic endopeptidase complex subunit C2 NU PROS 30 PROS-30 PROS30 Proteasome (prosome macropain) subunit alpha type 1 Proteasome alpha 1 subunit Proteasome component C2 Proteasome nu chain Proteasome subunit alpha type 1 Proteasome subunit alpha type I Proteasome subunit alpha type-1 Proteasome subunit nu Protein P30 33K PSA1_HUMAN PSC 2 PSC2 PSMA 1 psmA1
Images
ET1706-19_1.jpg Fig1: Western blot analysis of PSMA1 on different lysates with Rabbit anti-PSMA1 antibody (ET1706-19) at 1/500 dilution.

Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: 293 cell lysate


Lysates/proteins at 10 µg/Lane.

Predicted band size: 30 kDa
Observed band size: 30 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-19) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature.
ET1706-19_2.jpg Fig2: Western blot analysis of PSMA1 on different lysates with Rabbit anti-PSMA1 antibody (ET1706-19) at 1/500 dilution.

Lane 1: PC-12 cell lysate
Lane 2: mouse spleen tissue lysate(20 µg/Lane)
Lane 3: rat spleen tissue lysate(20 µg/Lane)


Lysates/proteins at 10 µg/Lane.

Predicted band size: 30 kDa
Observed band size: 30 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-19) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature.
ET1706-19_3.jpg Fig3: Immunocytochemistry analysis of HepG2 cells labeling PSMA1 with Rabbit anti-PSMA1 antibody (ET1706-19) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMA1 antibody (ET1706-19) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-19_4.jpg Fig4: ICC staining of PSMA1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1706-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1706-19_5.jpg Fig5: Immunocytochemistry analysis of PC-3M cells labeling PSMA1 with Rabbit anti-PSMA1 antibody (ET1706-19) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMA1 antibody (ET1706-19) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-19_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-PSMA1 antibody (ET1706-19) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-19) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-19_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PSMA1 antibody (ET1706-19) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-19) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-19_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PSMA1 antibody (ET1706-19) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-19) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-19_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PSMA1 antibody (ET1706-19) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-19) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-19_10.jpg Fig10: Flow cytometric analysis of PSMA1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-19, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.