IKBKE Recombinant Rabbit Monoclonal Antibody [JU06-72]
cat.: ET1706-20
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU06-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 80 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IKBKE aa 26-75 / 716. |
| Positive control: | Daudi cell lysate, Raji cell lysate, MCF-7 cell lysate, Jurkat cell lysate, Hela, JAR, SK-Br-3, human kidney tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC IHC-P |
1:500-1:2,000 1:50-1:200 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: Q14164 Human |
| Alternative names: | I kappa B kinase epsilon I-kappa-B kinase epsilon IkBKE IKK related kinase epsilon IKK-E IKK-epsilon IKK-i IKKE IKKE_HUMAN IKKepsilon IKKI Inducible I kappa B kinase Inducible I kappa-B kinase Inducible IkappaB kinase Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon Inhibitor of nuclear factor kappa-B kinase subunit epsilon KIAA0151 MGC125294 MGC125295 MGC125297 |
Images
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Fig1:
Western blot analysis of IKBKE on different lysates with Rabbit anti-IKBKE antibody (ET1706-20) at 1/500 dilution. Lane 1: Daudi cell lysates Lane 2: Raji cell lysates Lane 3: MCF-7 cell lysates Lane 4: Jurkat cell lysates Lysates/proteins at 10 µg/Lane. Predicted band size: 80 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-20) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Hela cells labeling IKBKE with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilutionNuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of JAR cells labeling IKBKE with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of SK-Br-3 cells labeling IKBKE with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-20) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.