IKBKE Recombinant Rabbit Monoclonal Antibody [JU06-72]
cat.: ET1706-20
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU06-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IKBKE aa 26-75 / 716. |
| Positive control: | Human spleen tissue, human thymus tissue, HeLa cell lysate, Raji cell lysate, Daudi cell lysate, Hela, JAR, SK-Br-3. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC IHC-P |
1:500-1:2,000 1:50-1:200 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: Q14164 Human |
| Alternative names: | I kappa B kinase epsilon I-kappa-B kinase epsilon IkBKE IKK related kinase epsilon IKK-E IKK-epsilon IKK-i IKKE IKKE_HUMAN IKKepsilon IKKI Inducible I kappa B kinase Inducible I kappa-B kinase Inducible IkappaB kinase Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon Inhibitor of nuclear factor kappa-B kinase subunit epsilon KIAA0151 MGC125294 MGC125295 MGC125297 |
Images
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Fig1:
Western blot analysis of IKBKE on different lysates with Rabbit anti-IKBKE antibody (ET1706-20) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Raji cell lysate (20 µg/Lane) Lane 3: Daudi cell lysate (20 µg/Lane) Predicted band size: 80 kDa Observed band size: 75 kDa Exposure time: Lane 1: 3 minutes; Lane 2-3: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-20) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-IKBKE antibody (ET1706-20) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-20) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-IKBKE antibody (ET1706-20) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-20) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of Hela cells labeling IKBKE with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilutionNuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunocytochemistry analysis of JAR cells labeling IKBKE with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig6:
Immunocytochemistry analysis of SK-Br-3 cells labeling IKBKE with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IKBKE antibody (ET1706-20) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.