DDB1 Recombinant Rabbit Monoclonal Antibody [JU32-35]
cat.: ET1706-22
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | JU32-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 127 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DDB1 aa 1040-1140 / 1,140. |
| Positive control: | MCF7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, C6 cell lysate, PC-12, human kidney tissue, mouse brain tissue, rat esophagus tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP FC |
1:2,000 1:50-1:200 1:50-1:200 1:1,000 1:10-1:50 1:1,000 |
| Uniprot #: | SwissProt: Q16531 Human | Q3U1J4 Mouse | Q9ESW0 Rat |
| Alternative names: | Damage specific DNA binding protein 1 Damage-specific DNA-binding protein 1 DDB 1 DDB p127 subunit Ddb1 DDB1_HUMAN DDBa DNA damage binding protein 1 DNA damage-binding protein 1 DNA damage-binding protein a HBV X-associated protein 1 UV damaged DNA binding factor UV damaged DNA binding protein 1 UV DDB 1 UV DDB1 UV-damaged DNA-binding factor UV-damaged DNA-binding protein 1 UV-DDB 1 X associated protein 1 XAP 1 XAP-1 XAP1 Xeroderma pigmentosum group E complementing protein Xeroderma pigmentosum group E-complementing protein XPCe XPE XPE BF XPE binding factor XPE-BF XPE-binding factor |
Images
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Fig1:
Western blot analysis of DDB1 on different lysates with Rabbit anti-DDB1 antibody (ET1706-22) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-22) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of PC-12 cells labeling DDB1 with Rabbit anti-DDB1 antibody (ET1706-22) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDB1 antibody (ET1706-22) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-DDB1 antibody (ET1706-22) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-22) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-DDB1 antibody (ET1706-22) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-22) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-DDB1 antibody (ET1706-22) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-22) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.