SAMHD1 Recombinant Rabbit Monoclonal Antibody [JU56-04]
cat.: ET1706-24
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JU56-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 72 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human SAMHD1 aa 577-626 / 626.
Positive control: THP-1 cell lysates, HepG2, HUVEC, LOVO, human tonsil tissue tissue, human colon carcinoma tissue, human spleen tissue, K562.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:100-1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9Y3Z3 Human
Alternative names: CHBL2 DCIP Dendritic cell derived IFNG induced protein Dendritic cell-derived IFNG-induced protein Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 dNTPase HD domain containing 1 HDDC1 Mg11 Monocyte protein 5 MOP 5 MOP-5 MOP5 OTTHUMP00000030889 SAM domain and HD domain 1 SAM domain and HD domain containing protein 1 SAM domain and HD domain-containing protein 1 SAMH1_HUMAN Samhd1 SBBI88
Images
ET1706-24_1.jpg Fig1: Western blot analysis of SAMHD1 on THP-1 cell lysates with Rabbit anti-SAMHD1 antibody (ET1706-24) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 72 kDa
Observed band size: 72 kDa

Exposure time: 30 seconds;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-24) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1706-24_2.jpg Fig2: Immunocytochemistry analysis of HepG2 cells labeling SAMHD1 with Rabbit anti-SAMHD1 antibody (ET1706-24) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SAMHD1 antibody (ET1706-24) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-24_3.jpg Fig3: Immunocytochemistry analysis of HUVEC cells labeling SAMHD1 with Rabbit anti-SAMHD1 antibody (ET1706-24) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SAMHD1 antibody (ET1706-24) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-24_4.jpg Fig4: Immunocytochemistry analysis of LOVO cells labeling SAMHD1 with Rabbit anti-SAMHD1 antibody (ET1706-24) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SAMHD1 antibody (ET1706-24) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-24_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue tissue with Rabbit anti-SAMHD1 antibody (ET1706-24) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-24) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-24_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SAMHD1 antibody (ET1706-24) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-24) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-24_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-SAMHD1 antibody (ET1706-24) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-24) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-24_8.jpg Fig8: Flow cytometric analysis of SAMHD1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-24, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.