NUP153 Recombinant Rabbit Monoclonal Antibody [JU40-41]
cat.: ET1706-25
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | JU40-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 154 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NUP153 aa 51-100 / 1,475. |
| Positive control: | K562 cell lysate, HepG2 cell lysate, A431, Hela, HepG2. |
| Subcellular location: | Nucleus, nuclear pore complex. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue FC |
1:500-1:2,000 1:100-1:500 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: P49790 Human |
| Alternative names: | 153 kDa nucleoporin HNUP153 N153 NU153_HUMAN Nuclear pore complex protein hnup153 Nuclear pore complex protein Nup153 Nucleoporin 153kDa Nucleoporin Nup153 Nup 153 Nup153 |
Images
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Fig1:
Western blot analysis of NUP153 on different lysates with Rabbit anti-NUP153 antibody (ET1706-25) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-25) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A431 cells labeling NUP153 with Rabbit anti-NUP153 antibody (ET1706-25) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NUP153 antibody (ET1706-25) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of Hela cells labeling NUP153 with Rabbit anti-NUP153 antibody (ET1706-25) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NUP153 antibody (ET1706-25) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
|
Fig4:
Immunocytochemistry analysis of HepG2 cells labeling NUP153 with Rabbit anti-NUP153 antibody (ET1706-25) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NUP153 antibody (ET1706-25) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.