NUP153 Recombinant Rabbit Monoclonal Antibody [JU40-41]
cat.: ET1706-25
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JU40-41 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 154 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NUP153 aa 51-100 / 1,475. |
| Positive control: | HeLa cell lysate, K-562 cell lysate. |
| Subcellular location: | Nucleus, nuclear pore complex. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: P49790 Human |
| Alternative names: | 153 kDa nucleoporin HNUP153 N153 NU153_HUMAN Nuclear pore complex protein hnup153 Nuclear pore complex protein Nup153 Nucleoporin 153kDa Nucleoporin Nup153 Nup 153 Nup153 |
Images
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Fig1:
Western blot analysis of NUP153 on different lysates with Rabbit anti-NUP153 antibody (ET1706-25) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-25) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.