Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JU40-41 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 154 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human NUP153 aa 51-100 / 1,475. |
Positive control: | HeLa cell lysate, K-562 cell lysate. |
Subcellular location: | Nucleus, nuclear pore complex. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: P49790 Human |
Alternative names: | 153 kDa nucleoporin HNUP153 N153 NU153_HUMAN Nuclear pore complex protein hnup153 Nuclear pore complex protein Nup153 Nucleoporin 153kDa Nucleoporin Nup153 Nup 153 Nup153 |
Fig1:
Western blot analysis of NUP153 on different lysates with Rabbit anti-NUP153 antibody (ET1706-25) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-25) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |