NUP153 Recombinant Rabbit Monoclonal Antibody [JU40-41]
cat.: ET1706-25
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JU40-41
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 154 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human NUP153 aa 51-100 / 1,475.
Positive control: HeLa cell lysate, K-562 cell lysate.
Subcellular location: Nucleus, nuclear pore complex.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: P49790 Human
Alternative names: 153 kDa nucleoporin HNUP153 N153 NU153_HUMAN Nuclear pore complex protein hnup153 Nuclear pore complex protein Nup153 Nucleoporin 153kDa Nucleoporin Nup153 Nup 153 Nup153
Images
ET1706-25_1.jpg Fig1: Western blot analysis of NUP153 on different lysates with Rabbit anti-NUP153 antibody (ET1706-25) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 154 kDa
Observed band size: 154 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-25) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.