HSD11B1 Recombinant Rabbit Monoclonal Antibody [JU18-39]
cat.: ET1706-27
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU18-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HSD11B1 aa 1-50 / 292. |
| Positive control: | Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, mouse liver tissue. |
| Subcellular location: | Endoplasmic reticulum, Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P28845 Human | P50172 Mouse | P16232 Rat |
| Alternative names: | 11 beta HSD 1 11 beta HSD1 11 beta hydroxysteroid dehydrogenase 1 11 DH 11-beta hydroxysteroid dehydrogenase, type 1 11-beta-HSD1 11-beta-hydroxysteroid dehydrogenase 1 11-DH 11DH Corticosteroid 11 beta dehydrogenase isozyme 1 Corticosteroid 11-beta-dehydrogenase isozyme 1 CORTRD2 DHI1_HUMAN HDL HSD 11 HSD11 HSD11B HSD11B1 HSD11L Hydroxysteroid (11 beta) dehydrogenase Hydroxysteroid (11 beta) dehydrogenase 1 MGC13539 SDR26C1 short chain dehydrogenase/reductase family 26C, member 1 |
Images
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Fig1:
Western blot analysis of HSD11B1 on different lysates with Rabbit anti-HSD11B1 antibody (ET1706-27) at 1/2,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse brain tissue lysate (negative) Lane 3: Rat liver tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 32 kDa Observed band size: 35 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-27) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HSD11B1 antibody (ET1706-27) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-27) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HSD11B1 antibody (ET1706-27) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-27) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.