Apg3 Recombinant Rabbit Monoclonal Antibody [JU00-35]
cat.: ET1706-29
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU00-35 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Apg3 1-50 / 314. |
| Positive control: | K562 cell lysate, HL-60 cell lysate, Hela cell lysate, Jurkat cell lysate, human tonsil tissue, human thyroid gland tissue, human colon carcinoma tissue, mouse small intestine tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9NT62 Human | Q9CPX6 Mouse | Q6AZ50 Rat |
| Alternative names: | 2610016C12Rik Apg 3 APG3 APG3 autophagy 3 like APG3 like APG3, S. cerevisiae, homolog of APG3-like APG3L Apg3p ATG 3 ATG3 ATG3 autophagy related 3 homolog ATG3 autophagy related 3 homolog (S. cerevisiae) ATG3_HUMAN Autophagy 3, S. cerevisiae, homolog of Autophagy Apg3p/Aut1p like autophagy related 3 Autophagy related protein 3 Autophagy-related protein 3 DKFZp564M1178 FLJ22125 hApg3 MGC15201 OTTHUMP00000214547 OTTHUMP00000214548 PC3 96 Protein PC3-96 Ubiquitin-like-conjugating enzyme ATG3 |
Images
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Fig1:
Western blot analysis of Apg3 on different lysates with Rabbit anti-Apg3 antibody (ET1706-29) at 1/500 dilution. Lane 1: Mouse testis tissue (20 µg/Lane) Lane 2: K562 cell lysate (10 µg/Lane) Lane 3: HL-60 cell lysate (10 µg/Lane) Lane 4: Hela cell lysate (10 µg/Lane) Lane 5: Jurkat cell lysate (10 µg/Lane) Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-29) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Apg3 antibody (ET1706-29) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-29) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue with Rabbit anti-Apg3 antibody (ET1706-29) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-29) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Apg3 antibody (ET1706-29) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-29) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Apg3 antibody (ET1706-29) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-29) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.