SDHB Recombinant Rabbit Monoclonal Antibody [JU02-33]
cat.: ET1706-30
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU02-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SDHB aa 201-250 / 280. |
| Positive control: | HepG2 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, Rat liver tissue lysate, Human liver tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, human liver tissue, human colon carcinoma tissue. |
| Subcellular location: | Mitochondrion. Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P21912 Human | Q9CQA3 Mouse | P21913 Rat |
| Alternative names: | CWS2 DHSB_HUMAN FLJ92337 Ip Iron sulfur subunit Iron sulfur subunit of complex II Iron-sulfur subunit of complex II mitochondrial PGL 4 PGL4 SDH 1 SDH SDH1 SDH2 SDH2, homolog of SdhB SDHIP Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial Succinate dehydrogenase [ubiquinone] iron sulfur subunit Succinate dehydrogenase [ubiquinone] iron-sulfur subunit succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial Succinate Dehydrogenase 1 Iron Sulfur Subunit Succinate Dehydrogenase 2, S. cerevisiae, homolog of Succinate dehydrogenase complex iron sulfur subunit B Succinate dehydrogenase complex subunit B iron sulfur Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein succinate dehydrogenase complex, subunit B, iron sulfur (Ip) Succinate dehydrogenase iron sulfur protein |
Images
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Fig1:
All lanes: Western blot analysis of SDHB with anti-SDHB antibody [JU02-33] (ET1706-30) at 1:1,000 dilution. Lane 1: Wild-type Hela whole cell lysate. Lane 2: SDHB knockout Hela whole cell lysate. ET1706-30 was shown to specifically react with SDHB in wild-type Hela cells. No band was observed when SDHB knockout samples were tested. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-SDHB antibody (ET1706-30, 1/1,000) and Anti-GAPDH antibody (ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
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Fig2:
Western blot analysis of SDHB on different lysates with Rabbit anti-SDHB antibody (ET1706-30) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (40 µg/Lane) Lane 3: Rat spleen tissue lysate (40 µg/Lane) Lane 4: Rat liver tissue lysate (40 µg/Lane) Lane 5: Human liver tissue lysate (40 µg/Lane) Predicted band size: 32 kDa Observed band size: 27 kDa Exposure time: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-30) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SDHB antibody (ET1706-30) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-30) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SDHB antibody (ET1706-30) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-30) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SDHB antibody (ET1706-30) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-30) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.