Involucrin Recombinant Rabbit Monoclonal Antibody [JU30-27]
cat.: ET1706-32
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JU30-27 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Involucrin aa 131-180 / 585. |
| Positive control: | A431 (Human epidermoid carcinoma skin squamous cell) cell lysates, human lung carcinoma tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P07476 Human |
| Alternative names: | INVO_HUMAN Involucrin IVL |
Images
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Fig1:
Western blot analysis of Involucrin on A431 (Human epidermoid carcinoma skin squamous cell) cell lysate with Rabbit anti-Involucrin antibody (ET1706-32) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1706-32, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 68 kDa Observed band size: 120 kDa |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Involucrin antibody (ET1706-32) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-32) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.