Wnt5a Recombinant Rabbit Monoclonal Antibody [JU30-30]
cat.: ET1706-33
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU30-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Wnt5a aa 331-380 / 380. |
| Positive control: | HeLa cell lysates, human lung cancer tissue. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P41221 Human |
| Alternative names: | hWNT 5A hWNT5A Protein Wnt 5a Protein Wnt-5a Protein Wnt5a Wingless type MMTV integration site family member 5A Wnt 5a WNT 5A protein WNT 5A protein precursor WNT5A WNT5A protein precursor WNT5A_HUMAN |
Images
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Fig1:
Western blot analysis of Wnt5a on HeLa cell lysates with Rabbit anti-Wnt5a antibody (ET1706-33) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 45 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-33) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Wnt5a antibody (ET1706-33) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-33) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.