Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JU30-30 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 42 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Wnt5a aa 331-380 / 380. |
Positive control: | Hela cell lysates, PC-3M, human lung cancer tissue, SH-SY5Y. |
Subcellular location: | Secreted, extracellular space, extracellular matrix. |
Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:500-1:2,000 1:1,000 1:50 1:50-1:100 |
Uniprot #: | SwissProt: P41221 Human |
Alternative names: | hWNT 5A hWNT5A Protein Wnt 5a Protein Wnt-5a Protein Wnt5a Wingless type MMTV integration site family member 5A Wnt 5a WNT 5A protein WNT 5A protein precursor WNT5A WNT5A protein precursor WNT5A_HUMAN |
Fig1:
Western blot analysis of Wnt5a on Hela cell lysates with Rabbit anti-Wnt5a antibody (ET1706-33) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-33) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of PC-3M cells labeling Wnt5a with Rabbit anti-Wnt5a antibody (ET1706-33) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Wnt5a antibody (ET1706-33) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Wnt5a antibody (ET1706-33) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-33) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig4: Flow cytometric analysis of SH-SY5Y cells with Wnt5a antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |