Transglutaminase 2 Recombinant Rabbit Monoclonal Antibody [JU30-02]
cat.: ET1706-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JU30-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 77 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Transglutaminase 2 aa 578-627 / 687. |
| Positive control: | Mouse placenta tissue lysate, mouse lung tissue tissue lysate, HUVEC cell lysates, human placenta tissue. |
| Subcellular location: | Cytosol. Plasma Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P21980 Human | P21981 Mouse |
| Alternative names: | ALPHA SUBUNIT C polypeptide EC 2.3.2.13 epididymis secretory protein Li 45 G alpha h G[a]h Gh CLASS G ALPHA h GNAH GNAH G PROTEIN H POLYPEPTIDE HEL-S-45 Protein glutamine gamma glutamyltransferase 2 Protein-glutamine gamma-glutamyltransferase 2 TG 2 TG(C) TG2 TGase C TGase H TGase-2 TgaseII TGC TGC GUANINE NUCLEOTIDE BINDING PROTEIN TGM2 TGM2_HUMAN Tissue transglutaminase Transglutaminase 2 Transglutaminase 2 C polypeptide Transglutaminase C Transglutaminase H Transglutaminase-2 tTG tTGas |
Images
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Fig1:
All lanes: Western blot analysis of Transglutaminase 2 with anti-Transglutaminase 2 antibody [JU30-02] (ET1706-35) at 1:1,000 dilution. Lane 1: Wild-type MDCK whole cell lysate. Lane 2: TGM2 knockout MDCK whole cell lysate. ET1706-35 was shown to specifically react with Transglutaminase 2 in wild-type Hela cells. No band was observed when TGM2 knockout samples were tested. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Transglutaminase 2 antibody (ET1706-35, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
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Fig2:
Western blot analysis of Transglutaminase 2 on different lysates with Rabbit anti-Transglutaminase 2 antibody (ET1706-35) at 1/500 dilution. Lane 1: Mouse placenta tissue lysate Lane 2: Mouse lung tissue tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 77 kDa Observed band size: 77 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-35) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of Transglutaminase 2 on HUVEC cell lysates with Rabbit anti-Transglutaminase 2 antibody (ET1706-35) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 77 kDa Observed band size:77 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-35) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Transglutaminase 2 antibody (ET1706-35) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-35) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.