Transglutaminase 2 Recombinant Rabbit Monoclonal Antibody [JU30-02]
cat.: ET1706-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU30-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Transglutaminase 2 aa 578-627 / 687. |
| Positive control: | K-562 cell lysate, HeLa cell lysate, HUVEC cell lysate, Mouse placenta tissue lysate, human placenta tissue, mouse placenta tissue, rat placenta tissue. |
| Subcellular location: | Cytosol. Plasma Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200 |
| Uniprot #: | SwissProt: P21980 Human | P21981 Mouse Entrez Gene: 56083 Rat |
| Alternative names: | ALPHA SUBUNIT C polypeptide EC 2.3.2.13 epididymis secretory protein Li 45 G alpha h G[a]h Gh CLASS G ALPHA h GNAH GNAH G PROTEIN H POLYPEPTIDE HEL-S-45 Protein glutamine gamma glutamyltransferase 2 Protein-glutamine gamma-glutamyltransferase 2 TG 2 TG(C) TG2 TGase C TGase H TGase-2 TgaseII TGC TGC GUANINE NUCLEOTIDE BINDING PROTEIN TGM2 TGM2_HUMAN Tissue transglutaminase Transglutaminase 2 Transglutaminase 2 C polypeptide Transglutaminase C Transglutaminase H Transglutaminase-2 tTG tTGas |
Images
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Fig1:
All lanes: Western blot analysis of Transglutaminase 2 with anti-Transglutaminase 2 antibody [JU30-02] (ET1706-35) at 1:1,000 dilution. Lane 1: Wild-type MDCK whole cell lysate. Lane 2: TGM2 knockout MDCK whole cell lysate. ET1706-35 was shown to specifically react with Transglutaminase 2 in wild-type Hela cells. No band was observed when TGM2 knockout samples were tested. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Transglutaminase 2 antibody (ET1706-35, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
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Fig2:
Western blot analysis of Transglutaminase 2 on different lysates with Rabbit anti-Transglutaminase 2 antibody (ET1706-35) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HeLa cell lysate Lane 3: HUVEC cell lysate Lane 4: Mouse placenta tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 77 kDa Observed band size: 70-77 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-35) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-Transglutaminase 2 antibody (ET1706-35) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-35) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat placenta tissue with Rabbit anti-Transglutaminase 2 antibody (ET1706-35) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-35) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.