HDAC9 Recombinant Rabbit Monoclonal Antibody [JU30-44]
cat.: ET1706-36
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JU30-44 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 111 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HDAC9 aa 1-50 / 1,011. |
| Positive control: | RD cell lysate, K-562 cell lysate, K-562, A431, human brain tissue, human bladder cancer tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:2,000 1:50-1:200 1:50-1:200 1:500-1:2,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9UKV0 Human |
| Alternative names: | HD 7 HD 7B HD 9 HD7 HD7B HD9 HDAC 7 HDAC 7B HDAC 9 HDAC 9B HDAC 9FL HDAC HDAC7 HDAC7B HDAC9 HDAC9_HUMAN HDAC9B HDAC9FL HDRP Histone deacetylase 4/5 related protein Histone deacetylase 7 Histone deacetylase 7B Histone deacetylase 9 Histone deacetylase 9A Histone deacetylase related protein Histone deacetylase-related protein KIAA0744 MEF2 interacting transcription repressor MITR MEF2 interacting transcription repressor protein MEF2-interacting transcription repressor MITR MITR |
Images
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Fig1:
Western blot analysis of HDAC9 on different lysates with Rabbit anti-HDAC9 antibody (ET1706-36) at 1/2,000 dilution. Lane 1: RD cell lysate Lane 2: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 111 kDa Observed band size: 160 kDa Exposure time: 1 minute 18 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-36) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of K-562 cells labeling HDAC9 with Rabbit anti-HDAC9 antibody (ET1706-36) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HDAC9 antibody (ET1706-36) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3: ICC staining of HDAC9 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1706-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-HDAC9 antibody (ET1706-36) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-36) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue with Rabbit anti-HDAC9 antibody (ET1706-36) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-36) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.