Mad2L2 Recombinant Rabbit Monoclonal Antibody [JU99-23]
cat.: ET1706-38
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JU99-23
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 24 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Mad2L2 aa 162-211 / 211.
Positive control: SiHa cell lysates, K562 cell lysates, rat lung tissue, mouse colon tissue, human tonsil tissue, Hela.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IP
1:500
1:50-1:200
1:50-1:100
1:10-1:50
Uniprot #: SwissProt: Q9UI95 Human | Q9D752 Mouse | D3Z8D9 Rat
Alternative names: Homolog of REV7 S cerevisiae hREV7 MAD2 (mitotic arrest deficient yeast, homolog) like 2 MAD2 homolog MAD2 like 2 MAD2 mitotic arrest deficient like 2 MAD2-like protein 2 MAD2B Mad2l2 MD2L2_HUMAN Mitotic Arrest Deficient 2 L2 Mitotic arrest deficient 2-like protein 2 Mitotic arrest deficient homolog like 2 Mitotic arrest deficient like 2 (yeast) Mitotic arrest deficient yeast homolog Mitotic spindle assembly checkpoint protein MAD2B Polymerase (DNA directed) zeta 2 accessory subunit POLZ2 REV 7 REV7 REV7 homolog Weakly similar to Mitotic MAD2 protein (S cerevisiae)
Images
ET1706-38_1.jpg Fig1: Western blot analysis of Mad2L2 on SiHa cell lysates with Rabbit anti-Mad2L2 antibody (ET1706-38) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 24 kDa
Observed band size: 24 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-38) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1706-38_2.jpg Fig2: Western blot analysis of Mad2L2 on K562 cell lysates with Rabbit anti-Mad2L2 antibody (ET1706-38) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 24 kDa
Observed band size: 24 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-38) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1706-38_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Mad2L2 antibody (ET1706-38) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-38) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-38_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Mad2L2 antibody (ET1706-38) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-38) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-38_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Mad2L2 antibody (ET1706-38) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-38) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-38_6.jpg Fig6: Flow cytometric analysis of Mad2L2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-38, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.