Anti-Mad2L2 antibody [JU99-23]
cat.: ET1706-38
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JU99-23
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 24 kDa
Isotype: IgG
Immunogen: Recombinant protein
Positive control: SiHa, rat lung tissue, mouse colon tissue, human tonsil tissue, Hela.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IP

1:500
1:50-1:200
1:50-1:100
1:10-1:50
Uniprot #: SwissProt: Q9UI95 Human | Q9D752 Mouse | D3Z8D9 Rat
Alternative names: Homolog of REV7 S cerevisiae hREV7 MAD2 (mitotic arrest deficient yeast, homolog) like 2 MAD2 homolog MAD2 like 2 MAD2 mitotic arrest deficient like 2 MAD2-like protein 2 MAD2B Mad2l2 MD2L2_HUMAN Mitotic Arrest Deficient 2 L2 Mitotic arrest deficient 2-like protein 2 Mitotic arrest deficient homolog like 2 Mitotic arrest deficient like 2 (yeast) Mitotic arrest deficient yeast homolog Mitotic spindle assembly checkpoint protein MAD2B Polymerase (DNA directed) zeta 2 accessory subunit POLZ2 REV 7 REV7 REV7 homolog Weakly similar to Mitotic MAD2 protein (S cerevisiae)
Images
ET1706-38_1.jpg Fig1: Western blot analysis of Mad2L2 on SiHa cell using anti-Mad2L2 antibody at 1/500 dilution.
ET1706-38_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.
ET1706-38_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.
ET1706-38_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.
ET1706-38_5.jpg Fig5: Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.