WASL Recombinant Rabbit Monoclonal Antibody [JU07-31]
cat.: ET1706-39
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JU07-31
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 55 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human WASL aa 401-450 / 505.
Positive control: Human serum, SH-SY5Y, SK-Br-3, human colon cancer tissue, human kidney tissue, human spleen tissue, mouse colon tissue, mouse brain tissue, K562.
Subcellular location: Cytoplasm, cytoskeleton. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O00401 Human | Q91YD9 Mouse
Alternative names: WASP family, verprolin homology domain-containing protein 1 WASP family member 1 FLJ31482 homology of dictyostelium scar 1 KIAA0269 Protein WAVE-1 v Protein WAVE1 scar, Dictyostelium, homology of, 1 SCAR1 Similar to a plant extensin like protein Verprolin homology domain containing protein 1 Verprolin homology domain-containing protein 1 WAS protein family, member 1 Wasf1 WASF1_HUMAN WASL WASP family 1 WASP family protein member 1 WASP family, verprolin homology domain-containing protein WAVE WAVE1 Wiskott Aldrich syndrome protein family member 1 Wiskott-Aldrich syndrome protein family member 1
Images
ET1706-39_1.jpg Fig1: Western blot analysis of WASL on mouse Human serum lysates using anti-Ubiquitin antibody at 1/500 dilution.
ET1706-39_2.jpg Fig2: ICC staining WASL in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1706-39_3.jpg Fig3: ICC staining WASL in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1706-39_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-WASL antibody. Counter stained with hematoxylin.
ET1706-39_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-WASL antibody. Counter stained with hematoxylin.
ET1706-39_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-WASL antibody. Counter stained with hematoxylin.
ET1706-39_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-WASL antibody. Counter stained with hematoxylin.
ET1706-39_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-WASL antibody. Counter stained with hematoxylin.
ET1706-39_9.jpg Fig9: Flow cytometric analysis of K562 cells with WASL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.