Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JU07-31 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 55 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human WASL aa 401-450 / 505. |
Positive control: | Human serum, SH-SY5Y, SK-Br-3, human colon cancer tissue, human kidney tissue, human spleen tissue, mouse colon tissue, mouse brain tissue, K562. |
Subcellular location: | Cytoplasm, cytoskeleton. Nucleus. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: O00401 Human | Q91YD9 Mouse |
Alternative names: | WASP family, verprolin homology domain-containing protein 1 WASP family member 1 FLJ31482 homology of dictyostelium scar 1 KIAA0269 Protein WAVE-1 v Protein WAVE1 scar, Dictyostelium, homology of, 1 SCAR1 Similar to a plant extensin like protein Verprolin homology domain containing protein 1 Verprolin homology domain-containing protein 1 WAS protein family, member 1 Wasf1 WASF1_HUMAN WASL WASP family 1 WASP family protein member 1 WASP family, verprolin homology domain-containing protein WAVE WAVE1 Wiskott Aldrich syndrome protein family member 1 Wiskott-Aldrich syndrome protein family member 1 |
Fig1: Western blot analysis of WASL on mouse Human serum lysates using anti-Ubiquitin antibody at 1/500 dilution. | |
Fig2: ICC staining WASL in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining WASL in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-WASL antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-WASL antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-WASL antibody. Counter stained with hematoxylin. | |
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-WASL antibody. Counter stained with hematoxylin. | |
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-WASL antibody. Counter stained with hematoxylin. |
Fig9: Flow cytometric analysis of K562 cells with WASL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |