Citrate synthetase Recombinant Rabbit Monoclonal Antibody [JU80-30]
cat.: ET1706-40
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JU80-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Citrate synthetase aa 417-466 / 466. |
| Positive control: | Rat kidney tissue lysate, Hela cell lysate, mouse brain tissue lysate, rat epididymis tissue, human tonsil tissue, human kidney tissue, 293T. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:2,000 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: O75390 Human | Q9CZU6 Mouse | Q8VHF5 Rat |
| Alternative names: | CISY_HUMAN Citrate synthase Citrate synthase, mitochondrial citrate synthetase Cs EC 2.3.3 EC 2.3.3.1 |
Images
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Fig1:
Western blot analysis of Citrate synthetase on different lysates with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Citrate synthetase KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 51 kDa Observed band size: 45 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-40) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Citrate synthetase on different lysates with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/1,000 dilution. Lane 1: Rat kidney tissue lysates (20 µg/Lane) Lane 2: Hela cell lysates Lane 3: Mouse brain tissue lysates (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-40) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-40) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-40) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-40) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of 293T cells with Citrate synthetase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.