Staufen Recombinant Rabbit Monoclonal Antibody [JU30-85]
cat.: ET1706-41
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, FC
Clonality: Monoclonal
Clone number: JU30-85
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 63 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Staufen aa 528-577 / 577.
Positive control: K562 cell lysates, LOVO cell lysates, HepG2, LOVO, SH-SY5Y
Subcellular location: Cytoplasm. Rough endoplasmic reticulum.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  FC
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O95793 Human | Q9Z108 Mouse
Entrez Gene: 84496 Rat
Alternative names: Double stranded RNA binding protein Staufen homolog 1 Double stranded RNA binding protein Staufen homolog Double-stranded RNA-binding protein Staufen homolog 1 FLJ25010 MGC124588 PPP1R150 STAU STAU1 STAU1_HUMAN staufen Staufen RNA binding protein (Drosophila) Staufen RNA binding protein homolog 1 Staufen, Drosophila, homolog of, 1 Staufen, RNA binding protein, homolog 1 (Drosophila) staufen-like
Images
ET1706-41_1.jpg Fig1: Western blot analysis of Staufen on different lysates with Rabbit anti-Staufen antibody (ET1706-41) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Staufen KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 63 kDa
Observed band size: 63/55 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-41) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1706-41_2.jpg Fig2: Western blot analysis of Staufen on K562 cell lysates with Rabbit anti-Staufen antibody (ET1706-41) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 63 kDa
Observed band size: 53 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-41) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1706-41_3.jpg Fig3: Western blot analysis of Staufen on LOVO cell lysates with Rabbit anti-Staufen antibody (ET1706-41) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 63 kDa
Observed band size: 53 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-41) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1706-41_4.jpg Fig4: Immunocytochemistry analysis of HepG2 cells labeling Staufen with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-41_5.jpg Fig5: Immunocytochemistry analysis of LOVO cells labeling Staufen with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-41_6.jpg Fig6: Immunocytochemistry analysis of SH-SY5Y cells labeling Staufen with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-41_7.jpg Fig7: Flow cytometric analysis of Staufen was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-41, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.