Staufen Recombinant Rabbit Monoclonal Antibody [JU30-85]
cat.: ET1706-41
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | JU30-85 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 63 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Staufen aa 528-577 / 577. |
| Positive control: | K562 cell lysates, LOVO cell lysates, HepG2, LOVO, SH-SY5Y |
| Subcellular location: | Cytoplasm. Rough endoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O95793 Human | Q9Z108 Mouse Entrez Gene: 84496 Rat |
| Alternative names: | Double stranded RNA binding protein Staufen homolog 1 Double stranded RNA binding protein Staufen homolog Double-stranded RNA-binding protein Staufen homolog 1 FLJ25010 MGC124588 PPP1R150 STAU STAU1 STAU1_HUMAN staufen Staufen RNA binding protein (Drosophila) Staufen RNA binding protein homolog 1 Staufen, Drosophila, homolog of, 1 Staufen, RNA binding protein, homolog 1 (Drosophila) staufen-like |
Images
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Fig1:
Western blot analysis of Staufen on K562 cell lysates with Rabbit anti-Staufen antibody (ET1706-41) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 63 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-41) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Staufen on LOVO cell lysates with Rabbit anti-Staufen antibody (ET1706-41) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 63 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-41) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunocytochemistry analysis of HepG2 cells labeling Staufen with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of LOVO cells labeling Staufen with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunocytochemistry analysis of SH-SY5Y cells labeling Staufen with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Staufen antibody (ET1706-41) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig6: Flow cytometric analysis of Staufen was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-41, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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