Alpha Fodrin Recombinant Rabbit Monoclonal Antibody [JU32-09]
cat.: ET1706-44
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU32-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 285 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Alpha Fodrin aa 46-95 / 2,472. |
| Positive control: | Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, mouse brain tissue lysate, human liver carcinoma tissue, human pancreas tissue, mouse cerebellum tissue. |
| Subcellular location: | Cytoskeleton. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q13813 Human | P16546 Mouse | P16086 Rat |
| Alternative names: | (ALPHA)II-SPECTRIN Alpha II Spectrin Alpha-II spectrin brain EIEE5 FLJ17738 FLJ44613 Fodrin alpha chain Fodrin, alpha NEAS Non erythrocytic spectrin alpha non-erythroid alpha chain SPECA Spectrin alpha chain Spectrin alpha chain brain Spectrin alpha non erythrocytic 1 Spectrin Spectrin non erythroid alpha chain Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) Spectrin, nonerythroid, alpha subunit Spna2 SPTA 2 SPTA2 SPTA2_HUMAN SPTAN 1 SPTAN1 |
Images
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Fig1:
Western blot analysis of Alpha Fodrin on different lysates with Rabbit anti-Alpha Fodrin antibody (ET1706-44) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lane 4:Rat brain tissue lysate(20 µg/Lane) Lane 5:Mouse brain tissue lysate(20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 285 kDa Observed band size: 285/150 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-44) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Alpha Fodrin antibody (ET1706-44) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-44) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Alpha Fodrin antibody (ET1706-44) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-44) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Alpha Fodrin antibody (ET1706-44) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-44) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.