Anti-TCTP antibody [JU13-32]
cat.: ET1706-48
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JU13-32
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 20 kDa
Isotype: IgG
Immunogen: Recombinant protein
Positive control: Mouse placenta tissue lysate, mouse testis tissue, Hela, A431, MCF-7, LOVO, SH-SY5Y, human colon cancer tissue, THP-1.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: P13693(Human) P63028(Mouse) P63029(Rat)
Alternative names: FLJ27337 antibody Fortilin antibody Histamine releasing factor antibody Histamine-releasing factor antibody HRF antibody Immunoglobulin E-dependent antibody p02 antibody p23 antibody TCTP antibody TCTP_HUMAN antibody tpt1 antibody Translationally controlled tumor protein antibody Translationally-controlled tumor protein antibody Tumor protein translationally controlled 1 antibody tumor protein, translationally-controlled 1 antibody
Images
ET1706-48_1.jpg Fig1: Western blot analysis of TCTP on different lysates using anti-TCTP antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse placenta tissue
Lane 2: Mouse testis tissue
Lane 3: Hela
Lane 4: A431
Lane 5: MCF-7
ET1706-48_2.jpg Fig2: ICC staining TCTP in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1706-48_3.jpg Fig3: ICC staining TCTP in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1706-48_4.jpg Fig4: ICC staining TCTP in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1706-48_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TCTP antibody. Counter stained with hematoxylin.
ET1706-48_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TCTP antibody. Counter stained with hematoxylin.
ET1706-48_7.jpg Fig7: Flow cytometric analysis of THP-1 cells with TCTP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.