TCTP Recombinant Rabbit Monoclonal Antibody [JU13-32]
cat.: ET1706-48
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JU13-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TCTP aa 35-84 / 172. |
| Positive control: | Mouse placenta tissue lysate, mouse testis tissue lysate, Hela cell lysate, A431 cell lysate, MCF-7 cell lysate, A431, LOVO, SH-SY-5Y, human colon carcinoma tissue, mouse testis tissue, THP-1. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P13693 Human | P63028 Mouse | P63029 Rat |
| Alternative names: | FLJ27337 Fortilin Histamine releasing factor Histamine-releasing factor HRF Immunoglobulin E-dependent p02 p23 TCTP TCTP_HUMAN tpt1 Translationally controlled tumor protein Translationally-controlled tumor protein Tumor protein translationally controlled 1 tumor protein, translationally-controlled 1 |
Images
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Fig1:
Western blot analysis of TCTP on different lysates with Rabbit anti-TCTP antibody (ET1706-48) at 1/500 dilution. Lane 1: Mouse placenta tissue lysate(20 µg/Lane) Lane 2: Mouse testis tissue lysate(20 µg/Lane) Lane 3: Hela cell lysate Lane 4: A431 cell lysate Lane 5: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-48) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A431 cells labeling TCTP with Rabbit anti-TCTP antibody (ET1706-48) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TCTP antibody (ET1706-48) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of LOVO cells labeling TCTP with Rabbit anti-TCTP antibody (ET1706-48) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TCTP antibody (ET1706-48) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of SH-SY-5Y cells labeling TCTP with Rabbit anti-TCTP antibody (ET1706-48) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TCTP antibody (ET1706-48) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-TCTP antibody (ET1706-48) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-48) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-TCTP antibody (ET1706-48) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-48) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Flow cytometric analysis of TCTP was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-48, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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