TCTP Recombinant Rabbit Monoclonal Antibody [JU13-32]
cat.: ET1706-48
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU13-32 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TCTP aa 35-84 / 172. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, THP-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human colon carcinoma tissue, mouse testis tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P13693 Human | P63028 Mouse | P63029 Rat |
| Alternative names: | FLJ27337 Fortilin Histamine releasing factor Histamine-releasing factor HRF Immunoglobulin E-dependent p02 p23 TCTP TCTP_HUMAN tpt1 Translationally controlled tumor protein Translationally-controlled tumor protein Tumor protein translationally controlled 1 tumor protein, translationally-controlled 1 |
Images
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Fig1:
Western blot analysis of TCTP on different lysates with Rabbit anti-TCTP antibody (ET1706-48) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-48) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-TCTP antibody (ET1706-48) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-48) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-TCTP antibody (ET1706-48) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-48) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.