Growth hormone receptor Recombinant Rabbit Monoclonal Antibody [JU01-34]
cat.: ET1706-49
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC, IF-Tissue
Clonality: Monoclonal
Clone number: JU01-34
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 72 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Growth hormone receptor aa 537-586 / 638.
Positive control: Rat brain tissue lysate, mouse brain tissue lysate, human brain tissue lysate, A549, SH-SY-5Y, mouse liver tissue, MCF-7.
Subcellular location: Secreted. Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
  IF-Tissue
1:500
1:50-1:200
1:50-1:1;000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: P10912 Human | P16882 Mouse | P16310 Rat
Alternative names: GH receptor GH-binding protein GHBP GHBP, included GHR GHR_HUMAN Growth hormone binding protein Growth hormone receptor Growth hormone receptor precursor Growth hormone-binding protein Growth hormone-binding protein, included Increased responsiveness to growth hormone, included Serum binding protein Serum-binding protein Somatotropin receptor
Images
ET1706-49_1.jpg Fig1: Western blot analysis of Growth hormone receptor on different lysates with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/500 dilution.

Lane 1: Rat brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Human brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 71 kDa
Observed band size: 140 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-49) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1706-49_2.jpg Fig2: Immunocytochemistry analysis of A549 cells labeling Growth hormone receptor with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-49_3.jpg Fig3: Immunocytochemistry analysis of HepG2 cells labeling Growth hormone receptor with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-49_4.jpg Fig4: Immunocytochemistry analysis of JAR cells labeling Growth hormone receptor with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-49_5.jpg Fig5: Immunocytochemistry analysis of SH-SY-5Y cells labeling Growth hormone receptor with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-49_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-49) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-49_7.jpg Fig7: Flow cytometric analysis of Growth hormone receptor was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-49, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1706-49_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-49) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-49_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-49) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-49_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-49) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-49_11.jpg Fig11: "Immunocytochemistry analysis of PC-12 cells labeling Growth hormone receptor with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Growth hormone receptor antibody (ET1706-49) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution."
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