HLA-DQA1 Recombinant Rabbit Monoclonal Antibody [JU17-34]
cat.: ET1706-51
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JU17-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HLA-DQA1 aa 205-254 / 254. |
| Positive control: | Rat lung tissue lysate, rat skin tissue lysate, mouse thymus tissue lysate, mouse spleen tissue lysate, Raji cell lysate, rat lung tissue, human tonsil tissue, mouse colon tissue. |
| Subcellular location: | Cell membrane. Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000-1:5,000 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: P01909 Human |
| Alternative names: | CD CELIAC1 DC 1 alpha chain DC alpha DC-1 alpha chain DC-alpha DC1, included DQ alpha 1 chain DQ-A1 DQ-DRW9 alpha chain DQA1_HUMAN FLJ27088 FLJ27328 Gluten-sensitive enteropathy (celiac disease) GSE HLA class II histocompatibility antigen HLA class II histocompatibility antigen, DQ alpha 1 chain HLA class II histocompatibility antigen, DQ(W3) alpha chain HLA-DCA HLA-DQA HLA-DQA1 HLA-DQA1 major histocompatibility complex, class II, DQ alpha 1 HLADC histocompatibility type Immune response antigens HIa, included leucocyte antigen DQA1 leukocyte antigen alpha chain LOC100133678 LOC100507686 LOC100509457 Major histocompatibility complex, class II, DQ alpha 1 MGC149527 MHC class II antigen MHC class II DQA1 MHC class II HLA-D alpha glycoprotein MHC class II HLA-DQ alpha 1 MHC class II surface glycoprotein MHC HLA-DQ alpha OTTHUMP00000029141 OTTHUMP00000176885 OTTHUMP00000178551 OTTHUMP00000178552 OTTHUMP0000023...... |
Images
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Fig1:
Western blot analysis of HLA-DQA1 on different lysates with Rabbit anti-HLA-DQA1 antibody (ET1706-51) at 1/500 dilution. Lane 1: Rat lung tissue lysate, 20 µg/Lane Lane 2: Rat skin tissue lysate, 20 µg/Lane Lane 3: Mouse thymus tissue lysate, 20 µg/Lane Lane 4: Mouse spleen tissue lysate, 20 µg/Lane Lane 5: Raji cell lysate, 10 µg/Lane Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-51) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-HLA-DQA1 antibody (ET1706-51) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-51) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HLA-DQA1 antibody (ET1706-51) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-51) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-HLA-DQA1 antibody (ET1706-51) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-51) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.