Nuclear Matrix Protein p84 Recombinant Rabbit Monoclonal Antibody [JU53-18]
cat.: ET1706-52
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JU53-18
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 76 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Nuclear Matrix Protein p84 aa 367-416 / 657.
Positive control: Hela cell lysate, A431 cell lysate, Mouse skeletal muscle lysate, PC-12 cell lysate, HUVEC, LOVO, rat testis tissue, rat brain tissue, human colon carcinoma tissue, human tonsil tissue, human lung carcinoma tissue.
Subcellular location: Nucleus speckle,Nucleus, nucleoplasm,Nucleus matrix,Cytoplasm
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q96FV9 Human | Q8R3N6 Mouse | P59924 Rat
Alternative names: hTREX84 Death domain containing protein p84N5 HPR 1 HPR1 hTREX84 Nuclear matrix protein p84 P84 p84N5 Tho 1 THO complex 1 THO complex subunit 1 Tho1 THOC 1 Thoc1 THOC1_HUMAN
Images
ET1706-52_1.jpg Fig1: Western blot analysis of Nuclear Matrix Protein p84 on different lysates with Rabbit anti-Nuclear Matrix Protein p84 antibody (ET1706-52) at 1/500 dilution.

Lane 1: Hela cell lysates
Lane 2: A431 cell lysates
Lane 3: Mouse skeletal muscle lysates(20 µg/Lane)
Lane 4: PC-12 cell lysates

Lysates/proteins at 10 µg/Lane.

Predicted band size: 76 kDa
Observed band size: 76 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-52) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature.
ET1706-52_2.jpg Fig2: Immunocytochemistry analysis of HUVEC cells labeling Nuclear Matrix Protein p84 with Rabbit anti-Nuclear Matrix Protein p84 antibody (ET1706-52) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nuclear Matrix Protein p84 antibody (ET1706-52) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-52_3.jpg Fig3: Immunocytochemistry analysis of LOVO cells labeling Nuclear Matrix Protein p84 with Rabbit anti-Nuclear Matrix Protein p84 antibody (ET1706-52) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nuclear Matrix Protein p84 antibody (ET1706-52) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-52_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Nuclear Matrix Protein p84 antibody (ET1706-52) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-52) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-52_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Nuclear Matrix Protein p84 antibody (ET1706-52) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-52) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-52_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Nuclear Matrix Protein p84 antibody (ET1706-52) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-52) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-52_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Nuclear Matrix Protein p84 antibody (ET1706-52) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-52) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-52_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Nuclear Matrix Protein p84 antibody (ET1706-52) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-52) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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