Anti-RbAP48 antibody [JU37-47]
cat.: ET1706-54
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JU37-47
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 48 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal Human RbAP48 .
Positive control: NIH-3T3, Hela, rat brain tissue, mouse testis tissue, MCF-7, SH-SY5Y, human tonsil tissue, human lung cancer tissue, human liver tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC
  IP

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
1:10-1:50
Uniprot #: Q09028(Human) Q60972(Mouse)
Alternative names: CAF I p48 antibody CAF-1 subunit C antibody CAF-I 48 kDa subunit antibody CAF-I p48 antibody Chromatin assembly factor 1 subunit C antibody Chromatin assembly factor I p48 subunit antibody Chromatin assembly factor/CAF 1 p48 subunit antibody Histone-binding protein RBBP4 antibody MSI1 protein homolog antibody Nucleosome-remodeling factor subunit RBAP48 antibody NURF55 antibody RB binding protein 4 chromatin remodeling factor antibody RbAp 48 antibody RBAP48 antibody RBBP-4 antibody RBBP4 antibody RBBP4_HUMAN antibody Retinoblastoma binding protein 4 antibody Retinoblastoma binding protein p48 antibody Retinoblastoma-binding protein 4 antibody Retinoblastoma-binding protein p48 antibody
Images
ET1706-54_1.jpg Fig1: Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.
Positive control:
Lane 1: NIH-3T3
Lane 2: Hela
Lane 3: Rat brain
Lane 4: Mouse testis
ET1706-54_2.jpg Fig2: ICC staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1706-54_3.jpg Fig3: ICC staining RbAP48 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1706-54_4.jpg Fig4: ICC staining RbAP48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1706-54_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
ET1706-54_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
ET1706-54_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
ET1706-54_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
ET1706-54_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
ET1706-54_10.jpg Fig10: Flow cytometric analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.