RbAP48 Recombinant Rabbit Monoclonal Antibody [JU37-47]
cat.: ET1706-54
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: JU37-47
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 48 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human RbAP48 aa 1-98 / 425.
Positive control: NIH-3T3 cell lysate, Hela cell lysate, rat brain tissue lysate, mouse testis tissue lysate, Hela, MCF-7, SH-SY5Y, rat brain tissue, human tonsil tissue, mouse testis tissue, human lung carcinoma tissue, human liver tissue,Hela.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
  IP
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
1:10-1:50
Uniprot #: SwissProt: Q09028 Human | Q60972 Mouse
Alternative names: CAF I p48 CAF-1 subunit C CAF-I 48 kDa subunit CAF-I p48 Chromatin assembly factor 1 subunit C Chromatin assembly factor I p48 subunit Chromatin assembly factor/CAF 1 p48 subunit Histone-binding protein RBBP4 MSI1 protein homolog Nucleosome-remodeling factor subunit RBAP48 NURF55 RB binding protein 4 chromatin remodeling factor RbAp 48 RBAP48 RBBP-4 RBBP4 RBBP4_HUMAN Retinoblastoma binding protein 4 Retinoblastoma binding protein p48 Retinoblastoma-binding protein 4 Retinoblastoma-binding protein p48
Images
ET1706-54_1.jpg Fig1: Western blot analysis of RbAP48 on different lysates with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/500 dilution.

Lane 1: NIH-3T3 cell lysates, 10 µg/Lane
Lane 2: Hela cell lysates, 10 µg/Lane
Lane 3: Rat brain tissue lysates, 20 µg/Lane
Lane 4: Mouse testis tissue lysates, 20 µg/Lane

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-54) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature.
ET1706-54_2.jpg Fig2: Immunocytochemistry analysis of Hela cells labeling RbAP48 with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-54_3.jpg Fig3: Immunocytochemistry analysis of MCF-7 cells labeling RbAP48 with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-54_4.jpg Fig4: Immunocytochemistry analysis of SH-SY5Y cells labeling RbAP48 with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1706-54_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-54) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-54_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-54) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-54_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-54) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-54_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-54) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-54_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-54) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1706-54_10.jpg Fig10: Flow cytometric analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.