EAAT3 Recombinant Rabbit Monoclonal Antibody [JU39-69]
cat.: ET1706-55
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JU39-69 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human EAAT3 aa 80-209 / 524. |
| Positive control: | Human brain tissue lysate, Human lung tissue lysate, SH-SY5Y, rat brain tissue, human tonsil tissue, human colon carcinoma tissue, human kidney tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:2,000 1:50-1:200 1:200 1:200-1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P43005 Human | P51906 Mouse | P51907 Rat |
| Alternative names: | EAA3_HUMAN EAAC 1 EAAC1 EAAT 3 Excitatory amino acid carrier 1 Excitatory amino acid carrier1 Excitatory amino acid transporter 3 Excitatory amino acid transporter3 Excitatory amino-acid carrier 1 GLUTAMATE TRANSPORTER, HIGH-AFFINITY MEAAC 1 MEAAC1 Neuronal and epithelial glutamate transporter REAAC 1 REAAC1 Slc1 a1 Slc1a 1 SLC1A1 Sodium dependent glutamate/aspartate transporter 3 Sodium-dependent glutamate/aspartate transporter 3 Solute carrier family 1 (neuronal / epithelial high affinity glutamate transporter, system Xag), member 1 SOLUTE CARRIER FAMILY 1 (NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER), MEMBER 1 Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 Solute carrier family 1 member 1 Solute carrier family 1, member 1 |
Images
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Fig1:
Western blot analysis of EAAT3 on different lysates with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Human lung tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 57 kDa Observed band size: 60 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-55) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling EAAT3 with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Flow cytometric analysis of SH-SY5Y cells labeling EAAT3. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1706-55, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-55) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.