ET1706-55

EAAT3 Recombinant Rabbit Monoclonal Antibody [JU39-69]

cat.: ET1706-55

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Rat, Mouse
Applications:	WB, IF-Cell, IF-Tissue, IHC-P
Clonality:	Monoclonal
Clone number:	JU39-69

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 57 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human EAAT3 aa 80-209 / 524.
Positive control:	Human lung tissue lysates, Hela, LOVO, SH-SY5Y, rat brain tissue, human tonsil tissue, human colon carcinoma tissue, human kidney tissue.
Subcellular location:	Cell membrane.
Recommended Dilutions: WB IF-Cell IF-Tissue IHC-P	1:500 1:50-1:200 1:50-1:200 1:50-1:200
Uniprot #:	SwissProt: P43005 Human \| P51906 Mouse \| P51907 Rat
Alternative names:	EAA3_HUMAN EAAC 1 EAAC1 EAAT 3 Excitatory amino acid carrier 1 Excitatory amino acid carrier1 Excitatory amino acid transporter 3 Excitatory amino acid transporter3 Excitatory amino-acid carrier 1 GLUTAMATE TRANSPORTER, HIGH-AFFINITY MEAAC 1 MEAAC1 Neuronal and epithelial glutamate transporter REAAC 1 REAAC1 Slc1 a1 Slc1a 1 SLC1A1 Sodium dependent glutamate/aspartate transporter 3 Sodium-dependent glutamate/aspartate transporter 3 Solute carrier family 1 (neuronal / epithelial high affinity glutamate transporter, system Xag), member 1 SOLUTE CARRIER FAMILY 1 (NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER), MEMBER 1 Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 Solute carrier family 1 member 1 Solute carrier family 1, member 1

Images

	Fig1: Western blot analysis of EAAT3 on human lung tissue lysates (20 µg/Lane) with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/500 dilution. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-55) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
	Fig2: Immunocytochemistry analysis of Hela cells labeling EAAT3 with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI.
	Fig3: Immunocytochemistry analysis of LOVO cells labeling EAAT3 with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

	Fig4: Immunocytochemistry analysis of SH-SY5Y cells labeling EAAT3 with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1706-55) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1706-55) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1706-55) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-EAAT3 antibody (ET1706-55) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1706-55) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.