Anti-Cullin 4a antibody [JU07-33]
cat.: ET7106-56
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IHC-P, FC
Clonality: Monoclonal
Clone number: JU07-33
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 88 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal Human Cullin 4a .
Positive control: Hela, A431, HUVEC, human liver cance tissue, human breast cancer tissue, human colon cancer tissue, human kidney tissue, human cervix tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: Q13619(Human)
Alternative names: 2810470J21Rik antibody AW495282 antibody CUL 4A antibody CUL-4A antibody Cul4a antibody Cul4a protein antibody CUL4A_HUMAN antibody Cullin-4A antibody MGC36573 antibody MGC64071 antibody
Images
ET7106-56_1.jpg Fig1: Western blot analysis of Cullin 4a on Hela cell lysates using anti-Cullin 4a antibody at 1/500 dilution.
ET7106-56_2.jpg Fig2: ICC staining Cullin 4a in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7106-56_3.jpg Fig3: ICC staining Cullin 4a in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7106-56_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.
ET7106-56_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.
ET7106-56_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.
ET7106-56_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.
ET7106-56_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.
ET7106-56_9.jpg Fig9: Flow cytometric analysis of Hela cells with Cullin 4a antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.