Anti-Cullin 4a antibody [JU07-33]
cat.: ET7106-56
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, ICC, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JU07-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/mL. |
| Purification: | Protein A purified. |
| Molecular weight: | 88 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal Human Cullin 4a . |
| Positive control: | Hela, A431, HUVEC, human liver cance tissue, human breast cancer tissue, human colon cancer tissue, human kidney tissue, human cervix tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB ICC IHC-P FC |
1:500-2,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | Q13619(Human) |
| Alternative names: | 2810470J21Rik antibody AW495282 antibody CUL 4A antibody CUL-4A antibody Cul4a antibody Cul4a protein antibody CUL4A_HUMAN antibody Cullin-4A antibody MGC36573 antibody MGC64071 antibody |
Images
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Fig1: Western blot analysis of Cullin 4a on Hela cell lysates using anti-Cullin 4a antibody at 1/500 dilution. |
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Fig2: ICC staining Cullin 4a in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Cullin 4a in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of Hela cells with Cullin 4a antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.