PERP Recombinant Rabbit Monoclonal Antibody [JU04-51]
cat.: ET7106-59
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU04-51 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PERP aa 144-193 / 193. |
| Positive control: | A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, HaCaT (Human immortalized keratinocyte) cell lysate, rat skin tissue lysate, human skin tissue, mouse small intestine tissue. |
| Subcellular location: | Cell junction. Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q96FX8 Human | Q9JK95 Mouse |
| Alternative names: | 1110017A08Rik an tibody dJ496H19.1 KCP 1 KCP-1 KCP1 Keratinocyte associated protein 1 Keratinocyte-associated protein 1 Keratinocytes associated protein 1 KRTCAP 1 KRTCAP1 p53 apoptosis effector related to PMP 22 p53 apoptosis effector related to PMP-22 p53 apoptosis effector related to PMP22 P53 induced protein PIGPC1 P53-induced protein PIGPC1 Perp PERP TP53 apoptosis effector PERP_HUMAN PIGPC 1 PIGPC1 RP3 496H19.1 THW TP53 apoptosis effector Transmembrane protein THW |
Images
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Fig1:
Western blot analysis of PERP on different lysates with Rabbit anti-PERP antibody (ET7106-59) at 1/5,000 dilution. Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 2: HaCaT (Human immortalized keratinocyte) cell lysate Lane 3: Rat skin tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7106-59, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 21 kDa Observed band size: 21 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-PERP antibody. Counter stained with hematoxylin. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PERP antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.