IL-13 Receptor alpha 1 Recombinant Rabbit Monoclonal Antibody [JU38-89]
cat.: ET7106-61
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU38-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL13 Receptor alpha 1 aa 1-127 / 427. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, HepG2, human liver tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:100-1:500 1:50-1:200 |
| Uniprot #: | SwissProt: P78552 Human |
| Alternative names: | bB128O4.2.1 (interleukin 13 receptor, alpha 1) Cancer/testis antigen 19 CD213a1 CD213A1 Antigen CT 19 CT19 I13R1_HUMAN IL 13 receptor subunit alpha 1 IL 13R alpha 1 IL 13R subunit alpha 1 IL 13Ra IL 13RA1 IL-13 receptor subunit alpha-1 IL-13R subunit alpha-1 IL-13R-alpha-1 IL-13RA1 IL13 receptor alpha 1 chain IL13R IL13RA Il13ra1 Interleukin 13 receptor alpha 1 Interleukin 13 receptor alpha 1 chain interleukin 13 receptor subunit alpha 1 Interleukin-13 receptor subunit alpha-1 NR 4 NR4 |
Images
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Fig1:
Western blot analysis of IL-13 Receptor alpha 1 on different lysates with Rabbit anti-IL-13 Receptor alpha 1 antibody (ET7106-61) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time: 10 second; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-61) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of IL-13 Receptor alpha 1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-61, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IL-13 Receptor alpha 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.