IGFBP2 Recombinant Rabbit Monoclonal Antibody [JU37-46]
cat.: ET7106-63
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JU37-46 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal Human IGFBP2 . |
| Positive control: | SH-SY5Y cell lysate, T-47D cell lysate, MCF7 cell lysate, BxPC-3 cell lysate, COS-1 cell lysate, human non-small cell lung carcinoma tissue, human tonsil tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P18065 Human |
| Alternative names: | BP 2 BP2 IBP 2 IBP-2 IBP2 IBP2_HUMAN IGF binding protein 2 IGF BP53 IGF-binding protein 2 IGFBP 2 IGFBP-2 IGFBP2 IGFBP53 Insulin like growth factor binding protein 2 36kDa Insulin like growth factor binding protein 2 Insulin like growth factor-binding protein 2 precursor Insulin-like growth factor-binding protein 2 |
Images
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Fig1:
Western blot analysis of IGFBP2 on different lysates with Rabbit anti-IGFBP2 antibody (ET7106-63) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: T-47D cell lysate Lane 3: MCF7 cell lysate Lane 4: BxPC-3 cell lysate Lane 5: JEG-3 cell lysate (low expression) Lane 6: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-63) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma tissue with Rabbit anti-IGFBP2 antibody (ET7106-63) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-63) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IGFBP2 antibody (ET7106-63) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-63) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.