ALDH1L1 Recombinant Rabbit Monoclonal Antibody [JU53-54]
cat.: ET7106-64
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JU53-54 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 99 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ALDH1L1 aa 155-375 . |
| Positive control: | A431, A549, LOVO, human liver tissue, human kidney tissue, mouse liver tissue, rat kidney tissue, rat brain tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IHC-Fr |
1:500-2,000 1:200-1:1,000 1:50-1:200 1:500 |
| Uniprot #: | SwissProt: O75891 Human | Q8R0Y6 Mouse | P28037 Rat |
| Alternative names: | 10 formyltetrahydrofolate dehydrogenase 10 FTHFDH 10-formyltetrahydrofolate dehydrogenase 10-FTHFDH AL1L1_HUMAN Aldehyde dehydrogenase 1 family member L1 Aldehyde dehydrogenase family 1 member L1 aldh1l1 Cytosolic 10-formyltetrahydrofolate dehydrogenase DKFZp781N0997 EC 1.5.1.6 FDH formyltetrahydrofolate dehydrogenase FTHFD OTTHUMP00000155221 OTTHUMP00000214696 OTTHUMP00000214700 OTTHUMP00000222724 |
Images
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Fig1:
Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7106-64, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7106-64, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling ALDH1L1 with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7106-64, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig6:
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling ALDH1L1 with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7106-64, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ALDH1L1 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ALDH1L1 antibody. Counter stained with hematoxylin. |
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Fig9: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ALDH1L1 antibody. Counter stained with hematoxylin. |
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Fig10: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ALDH1L1 antibody. Counter stained with hematoxylin. |
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Fig11: Western blot analysis of ALDH1L1 on mouse liver tissue lysates using anti-ALDH1L1 antibody at 1/500 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.